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A stable version of capillary electrophoresis for determining human hemoglobin chains aiming at the screening and diagnosis of thalassemia.
Analytical Methods ( IF 3.1 ) Pub Date : 2020-05-21 , DOI: 10.1039/d0ay00384k
Chao Guo 1, 2 , Qianchun Zhang 3 , Linchun Bao 4 , Hongliang Li 1, 2 , Qingfeng Zheng 1, 2 , Zhenpeng Guo 1, 2 , Yi Chen 1, 2, 5
Affiliation  

As a fast, high-performance and cost-effective separation technique, capillary electrophoresis (CE) is applicable to the screening and diagnosis of diseases such as thalassemia. However, it is often not preferred due to its unrepeatable and/or irreproducible migration times. Herein, we propose a stable version of CE that uses migration charge density instead of the migration time to plot the electropherogram. The peak position is now independent of the applied voltage or current and the capillary geometry and is also insensitive to temperature. Its applicability was demonstrated in the quantitative analysis of human hemoglobin. On a laboratory-built device, with a running buffer simply consisting of 3.0 M acetic acid and 0.1% (w/v) hydroxyethyl cellulose, it allows a direct injection of whole blood samples and all the concerned globin chains, α, β, Aγ and Gγ can be well separated in 15 minutes. The resolution of α/β, β/Aγ, and Aγ/Gγ reached 4.4, 3.1 and 5.3, respectively. The intra- and inter-day precisions for the peak position based on the migration charge densities were below 0.6%. Its diagnostic applicability was validated in the analysis of several real blood samples from newborns, children and adults, and its capacity was demonstrated to screen and define the type of thalassemia.

中文翻译:

一种稳定的毛细管电泳法,用于确定旨在筛选和诊断地中海贫血的人血红蛋白链。

毛细管电泳(CE)作为一种快速,高性能且具有成本效益的分离技术,适用于地中海贫血等疾病的筛查和诊断。然而,由于其不可重复和/或不可复制的迁移时间,通常不是优选的。在这里,我们提出了一个稳定的CE版本,它使用迁移电荷密度而不是迁移时间来绘制电泳图。现在,峰值位置与施加的电压或电流以及毛细管的几何形状无关,并且对温度不敏感。在人类血红蛋白的定量分析中证明了其适用性。在实验室制造的设备上,运行缓冲液仅包含3.0 M乙酸和0.1%(w / v)羟乙基纤维素,可直接注射全血样品和所有相关的球蛋白链,αβγg ^ γ可以很好地在15分钟内分离。的分辨率α / ββ /γ,和γ / g ^ γ达到4.4,分别为3.1和5.3。基于迁移电荷密度的峰值位置的日内和日间精度低于0.6%。它的诊断适用性已通过分析新生儿,儿童和成人的几种真实血液样本得到验证,并且具有筛查和定义地中海贫血类型的能力。
更新日期:2020-07-02
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