Deleted in malignant brain tumors 1 (DMBT1) is part of the innate immune system and is expressed on mucosal surfaces in various tissues throughout the human body. However, to date, the localization of DMBT1 has not been investigated systematically and comprehensively in normal human tissues. In this study, we analyzed the mRNA expression of DMBT1 in human tissue by quantitative real-time PCR and examined its localization and distribution in the tissue by immunohistochemical staining using the monoclonal DMBT1 antibody HYB213-6. Anti-ovalbumin was used as an isotype control. The highest level of mRNA expression of DMBT1 was found in the small intestine, and the expression level was high throughout the luminal digestive tract. The expression of DMBT1 was especially high in the luminal digestive tract and salivary glands. The lowest expression level was found in the spleen. Immunohistochemical staining showed a high expression level of DMBT1 on mucosal surfaces throughout the body. There was a clear correlation between the mRNA expression and immunohistochemical expression of DMBT1 in the tissue. DMBT1 is strongly expressed on mucosal surfaces and in salivary glands.

中文翻译：

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正常人组织中恶性脑肿瘤1中缺失的免疫组织化学定位。

在恶性脑肿瘤1（DMBT1）中缺失是先天免疫系统的一部分，在整个人体各种组织的粘膜表面表达。然而，迄今为止，尚未对DMBT1在正常人组织中的定位进行系统和全面的研究。在这项研究中，我们通过定量实时PCR分析了DMBT1在人体组织中的mRNA表达，并使用单克隆DMBT1抗体HYB213-6通过免疫组织化学染色检查了其在组织中的定位和分布。抗卵清蛋白用作同种型对照。在小肠中发现DMBT1的mRNA表达最高水平，并且在整个腔消化道中该表达水平高。DMBT1在腔消化道和唾液腺中的表达特别高。在脾脏中发现最低的表达水平。免疫组织化学染色显示，DMBT1在全身粘膜表面高表达。组织中DMBT1的mRNA表达与免疫组织化学表达之间存在明显的相关性。DMBT1在粘膜表面和唾液腺中强烈表达。