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Effect of early antibiotic intervention on specific bacterial communities and immune parameters in the small intestine of growing pigs fed different protein level diets.
Animal ( IF 3.7 ) Pub Date : 2020-05-21 , DOI: 10.1017/s1751731120001044
C J Zhang 1 , M Yu 1 , Y X Yang 1 , C L Mu 1 , Y Su 1 , W Y Zhu 1
Affiliation  

Antibiotics are designed to affect gut microbiota and subsequently gut homeostasis. However, limited information exists about short- and long-term effects of early antibiotic intervention (EAI) on gut homeostasis (especially for the small intestine) of pigs following antibiotic withdrawal. We investigated the impact of EAI on specific bacterial communities, microbial metabolites and mucosal immune parameters in the small intestine of later-growth-stage pigs fed with diets differing in CP levels. Eighteen litters of piglets were fed creep feed with or without antibiotics from day 7 to day 42. At day 42, pigs within each group were offered a normal- or low-CP diet. Five pigs per group were slaughtered at days 77 and 120. At day 77, EAI increased Enterobacteriaceae counts in the jejunum and ileum and decreased Bifidobacterium counts in the jejunum and ileum (P < 0.05). Moreover, tryptamine, putrescine, secretory immunoglobulin (Ig) A and IgG concentrations in the ileum and interleukin-10 (IL-10) mRNA and protein levels in the jejunum and ileum were decreased in pigs with EAI (P < 0.05). At day 120, EAI only suppressed Clostridium cluster XIVa counts in the jejunum and ileum (P < 0.05). These results suggest that EAI has a short-term effect on specific bacterial communities, amino acid decarboxylation and mucosal immune parameters in the small intestine (particularly in the ileum). At days 77 and 120, feeding a low-CP diet affected Bifidobacterium, Clostridium cluster IV, Clostridium cluster XIVa and Enterobacteriaceae counts in the jejunum or ileum (P < 0.05). Moreover, feeding a low-CP diet increased the concentrations of Igs in the jejunum and decreased pro-inflammatory cytokines levels in the jejunum and ileum (P < 0.05). At day 120, feeding a low-CP diet increased short-chain fatty acid concentrations, reduced ammonia and spermidine concentrations and up-regulated genes related to barrier function in the jejunum and ileum (P < 0.05). These results suggest that feeding a low-CP diet changes specific bacterial communities and intestinal metabolite concentrations and modifies mucosal immune parameters. These findings contribute to our understanding on the duration of the impact of EAI on gut homeostasis and may provide basis data for nutritional modification in young pigs after antibiotic treatment.



中文翻译:

早期抗生素干预对饲喂不同蛋白质水平日粮的生长猪小肠中特定细菌群落和免疫参数的影响。

抗生素旨在影响肠道菌群,进而影响肠道稳态。但是,关于早期抗生素干预(EAI)对停用抗生素后猪肠道稳态(特别是小肠)的短期和长期影响的信息有限。我们调查了饲喂不同CP水平日粮的成年猪的小肠中EAI对特定细菌群落,微生物代谢产物和粘膜免疫参数的影响。从第7天到第42天,给18窝仔猪饲喂含有或不含有抗生素的蠕变饲料。在第42天,给每组猪提供正常或低CP饮食。每组五只猪在第77天和第120天被宰杀。在第77天,EAI使空肠和回肠中肠杆菌科计数增加,并减少空肠和回肠中的双歧杆菌计数(P <0.05)。此外,EAI猪的回肠中色胺,腐胺,分泌性免疫球蛋白(Ig)A和IgG浓度以及空肠和回肠中白细胞介素10(IL-10)mRNA和蛋白水平降低(P <0.05)。在第120天,EAI仅抑制空肠和回肠中的梭状芽胞杆菌XIVa计数(P <0.05)。这些结果表明,EAI对小肠(特别是回肠)中的特定细菌群落,氨基酸脱羧和粘膜免疫参数具有短期影响。在第77天和第120天,喂养低CP饮食会影响双歧杆菌空肠或回肠中的梭状芽胞杆菌IV,梭状芽胞杆菌XIVa和肠杆菌科计数(P <0.05)。此外,喂养低CP饮食会增加空肠中Igs的浓度,并降低空肠和回肠中促炎性细胞因子的水平(P <0.05)。在第120天,饲喂低CP饮食会增加短链脂肪酸的浓度,降低氨和亚精胺的浓度,并导致空肠和回肠的屏障功能相关基因上调(P<0.05)。这些结果表明,饲喂低CP饮食会改变特定的细菌群落和肠道代谢产物浓度,并改变粘膜免疫参数。这些发现有助于我们了解EAI对肠道稳态的影响持续时间,并可能为抗生素治疗后的仔猪营养改良提供基础数据。

更新日期:2020-05-21
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