Abstract Context: There are numerous pharmacological activities for Ruta graveolens and its bioactive constituent, rutin, on learning and memory. Objective: This study aimed to examine the effect of R. graveolens and rutin on memory in rats. Materials and methods: In this study animals were treated with the hydroalcholic extract of R. graveolens and rutin by IP injection for 10 days. Behavioural and biochemical tests as well as HPLC analysis and antioxidant activity of extract have been evaluated. Results: R. graveolens extract and rutin significantly increased learning and improved spatial memory, as well as secondary latency; moreover, there were significant increases in the serum and brain antioxidant capacity as well as the level of TBARS in serum and brain tissues. Results also showed that R. graveolens has significant DPPH radical scavenging effect (IC50: 159.17 ± 1.56 μg/mL). The HPLC analysis of extract showed that caffeic acid (19.92 ± 0.01), rutin (40.15 ± 0.01), and apigenin (0.84 ± 0.01) mg/g of dry extract are the main components of the extract. Discussion and conclusion: Regarding the effects of R. graveolens extract and rutin on animal brain cells, memory function, and learning, additional studies, including clinical trials, might be beneficial in producing natural supplementary drugs from this herb.

中文翻译：

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芸香和芦丁，作为其主要化合物：研究它们对大鼠空间记忆和被动回避记忆的影响

摘要 背景：芸香及其生物活性成分芦丁对学习和记忆有许多药理活性。目的：本研究旨在检测 R.graveolens 和芦丁对大鼠记忆力的影响。材料和方法：在这项研究中，动物通过腹腔内注射用 R.graveolens 和芦丁的水醇提取物治疗 10 天。已经评估了提取物的行为和生化测试以及 HPLC 分析和抗氧化活性。结果：R.graveolens 提取物和芦丁显着增加学习和改善空间记忆，以及二次潜伏期；此外，血清和脑抗氧化能力以及血清和脑组织中的TBARS水平显着增加。结果还表明，R. Graveolens 具有显着的 DPPH 自由基清除作用（IC50：159.17 ± 1.56 μg/mL）。提取物的HPLC分析表明，咖啡酸（19.92±0.01）、芦丁（40.15±0.01）和芹菜素（0.84±0.01）mg/g干提取物是提取物的主要成分。讨论和结论：关于 R.graveolens 提取物和芦丁对动物脑细胞、记忆功能和学习的影响，包括临床试验在内的其他研究可能有助于从这种草药生产天然补充药物。