ABSTRACT

The viability testing in hemp seeds is a crucial step in its seed supply chain. Based on the legal status of hemp cultivation, each provincial area would benefit from the viability assessment, either by screening out dead seeds in legalized zones or by detecting the viable seeds in areas where hemp cultivation is not legalized. The routine procedure of assessing seed viability through germination tests takes a minimum of 7 days, which is practically not beneficial in the seed supply system. There is a need for developing a quick, reproducible, and fool-proof technique whereby seed lots can be rapidly screened, utilizing minimum resources. In this experiment, we have standardized a tetrazolium-based viability testing protocol which can identify the viability status of seed lots within 3 h.

摘要

大麻种子的活力测试是其种子供应链中的关键一步。根据大麻种植的法律地位，每个省区都将从可行性评估中受益，方法是筛选出合法区域的死种子，或者检测大麻种植未合法区域的可存活种子。通过发芽测试评估种子活力的常规程序至少需要 7 天，这实际上对种子供应系统没有好处。需要开发一种快速、可重复和简单的技术，从而可以利用最少的资源快速筛选种子批次。在本实验中，我们标准化了基于四唑的活力测试方案，可以在 3 小时内识别种子批次的活力状态。