Abstract Most studies dealing with monitoring the dynamics of biofilm formation use microbial suspensions at high concentrations. These conditions do not always represent food or water distribution systems. A continuous flow system capable of controlling the concentration of the microbial suspension stream from 104 to 106 CFU ml−1 is reported. Pseudomonas putida biofilms formed using 100-fold, 1,000-fold or 10,000-fold diluted bacterial suspensions were monitored in-line by electrochemical impedance spectroscopy (EIS) and total plate counts. Randles equivalent circuit model and a modified Randles model with biofilm elements were used to fit the EIS data. In Randles equivalent circuit, the charge transfer resistance decreased as the biofilm formed. The log colony counts of the biofilm correlated to the charge transfer resistance. In the biofilm model, the biofilm resistance and the double layer capacitance decreased as the biofilm formed. The log colony counts of the biofilm correlated to the biofilm resistance.

中文翻译：

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使用受控浓度的来自鸡胴体的恶臭假单胞菌并耦合电化学阻抗检测的连续流动系统形成生物膜

摘要 大多数涉及监测生物膜形成动态的研究使用高浓度的微生物悬浮液。这些条件并不总是代表食物或水分配系统。报道了一种能够将微生物悬浮液流的浓度从 104 控制到 106 CFU ml-1 的连续流系统。使用 100 倍、1,000 倍或 10,000 倍稀释的细菌悬浮液形成的恶臭假单胞菌生物膜通过电化学阻抗谱 (EIS) 和总板数进行在线监测。Randles 等效电路模型和带有生物膜元件的修正 Randles 模型用于拟合 EIS 数据。在兰德尔等效电路中，电荷转移电阻随着生物膜的形成而降低。生物膜的对数菌落计数与电荷转移电阻相关。在生物膜模型中，生物膜电阻和双层电容随着生物膜的形成而降低。生物膜的对数菌落计数与生物膜抗性相关。