Abstract A modified calibration curve method based on the combination of standard addition and traditional calibration curve methods was employed for the quantification of Prussian blue nanoparticles in rats in the presence of endogenous iron interferences. The assay was performed using inductively coupled plasma – optical emission spectrometry (ICP-OES) with yttrium as the internal standard, and validated in biomatrices including liver, heart, kidneys, lungs, spleen and whole blood. For the liver tissue, the intra-day and inter-day precision were 7.84% or less, and the accuracy was between 99.88% and 102.37%. For kidneys, lungs, heart, spleen, and blood, at each exo-additive level from 0.4 to 3.2 µg/mL, the intra-day accuracy was between 98.74% and 102.09%, and the intra-day precision was 6.17% or less. For the addition of no analyte, the intra-day accuracy was between 96.42% and 107.52%, and the intra-day precision was 15.67% or less. The stability was investigated at 25 °C for 24 h and 4 °C for 15 days in liver standard addition samples, and no significant degradation occurred under these conditions. This method was successfully applied to the preliminary pharmacokinetics and tissue distribution study of Prussian blue nanoparticles in rats. This approach may also be applied to the quantification of other ferruginous materials in the biological matrices with endogenous iron.

中文翻译：

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电感耦合等离子体发射光谱法 (ICP-OES) 测定存在内源性铁干扰的大鼠组织中的普鲁士蓝纳米颗粒

摘要 采用标准加入法与传统校准曲线法相结合的改进校准曲线法，用于在内源性铁干扰存在下对大鼠体内普鲁士蓝纳米颗粒的定量。该测定使用电感耦合等离子体 - 光学发射光谱法 (ICP-OES) 进行，以钇为内标，并在包括肝脏、心脏、肾脏、肺、脾脏和全血在内的生物基质中进行了验证。对于肝脏组织，日内和日间精密度为7.84%以下，准确度在99.88%至102.37%之间。对于肾脏、肺、心脏、脾脏和血液，在 0.4 至 3.2 µg/mL 的每个外添加剂水平下，日内准确度在 98.74% 至 102.09% 之间，日内精密度为 6.17% 或更低. 对于不添加分析物，日内准确率在96.42%～107.52%之间，日内准确率在15.67%以下。研究了肝脏标准添加样品在 25 °C 下 24 小时和 4 °C 下 15 天的稳定性，在这些条件下没有发生显着降解。该方法成功应用于普鲁士蓝纳米粒在大鼠体内的初步药代动力学和组织分布研究。这种方法也可用于定量分析生物基质中含有内源性铁的其他含铁物质。该方法成功应用于普鲁士蓝纳米粒在大鼠体内的初步药代动力学和组织分布研究。这种方法也可用于定量分析生物基质中含有内源性铁的其他含铁物质。该方法成功应用于普鲁士蓝纳米粒在大鼠体内的初步药代动力学和组织分布研究。这种方法也可用于定量分析生物基质中含有内源性铁的其他含铁物质。