Background: hydrogels prepared with natural inorganic excipients and spring waters are commonly used in medical hydrology. Design of these clay-based formulations continues to be a field scarcely addressed. Safety and wound healing properties of different fibrous nanoclay/spring water hydrogels were addressed. Methods: in vitro biocompatibility, by means of MTT assay, and wound healing properties were studied. Confocal Laser Scanning Microscopy was used to study the morphology of fibroblasts during the wound healing process. Results: all the ingredients demonstrated to be biocompatible towards fibroblasts. Particularly, the formulation of nanoclays as hydrogels improved biocompatibility with respect to powder samples at the same concentration. Spring waters and hydrogels were even able to promote in vitro fibroblasts motility and, therefore, accelerate wound healing with respect to the control. Conclusion: fibrous nanoclay/spring water hydrogels proved to be skin-biocompatible and to possess a high potential as wound healing formulations. Moreover, these results open new prospects for these ingredients to be used in new therapeutic or cosmetic formulations.

中文翻译：

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纳米粘土/泉水水凝胶的伤口愈合活性。

背景：用天然无机赋形剂和泉水制备的水凝胶通常用于医学水文学。这些基于粘土的配方的设计仍然是很少解决的领域。研究了不同纤维纳米粘土/泉水水凝胶的安全性和伤口愈合特性。方法：采用MTT法测定体外生物相容性，研究伤口愈合特性。共聚焦激光扫描显微镜用于研究伤口愈合过程中成纤维细胞的形态。结果：所有成分均证明对成纤维细胞具有生物相容性。特别地，相对于相同浓度的粉末样品，作为水凝胶的纳米粘土制剂改善了生物相容性。泉水和水凝胶甚至能够促进体外成纤维细胞的运动，因此，相对于对照，加速伤口愈合。结论：纤维纳米粘土/泉水水凝胶被证明具有皮肤生物相容性，并具有作为伤口愈合制剂的巨大潜力。而且，这些结果为将这些成分用于新的治疗或化妆品制剂中开辟了新的前景。