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Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom Chaetoceros Muelleri Using 13C-Isotope Labeling.
Biomolecules ( IF 5.5 ) Pub Date : 2020-05-21 , DOI: 10.3390/biom10050797
Marine Remize 1 , Frédéric Planchon 1 , Ai Ning Loh 2 , Fabienne Le Grand 1 , Antoine Bideau 1 , Nelly Le Goic 1 , Elodie Fleury 3 , Philippe Miner 3 , Rudolph Corvaisier 1 , Aswani Volety 4 , Philippe Soudant 1
Affiliation  

: The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of 13C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom Chaetoceros muelleri for 24 h. Chaetoceros muelleri preferentially and quickly incorporated 13C into C18 PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly 13C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids. The calculation of the enrichment percentage ratio of a fatty acid B over its suspected precursor A allowed us to suggest that the diatom produced 20:5n-3 (EPA) by a combination between the n-3 (via 18:4n-3) and n-6 (via 18:3n-6 and 20:4n-6) synthesis pathways as well as the alternative ω-3 desaturase pathway (via 20:4n-6). In addition, as FA from polar lipids were generally more enriched in 13C than FA from neutral lipids, particularly for 18:1n-9, 18:2n-6 and 18:3n-6, the existence of acyl-editing mechanisms and connectivity between polar and neutral lipid fatty acid pools were also hypothesized. Because 16:3n-4 and 20:5n-3 presented the same concentration and enrichment dynamics, a structural and metabolic link was proposed between these two PUFAs in C. muelleri.

中文翻译:

使用13C同位素标记研究硅藻Chaelleroceros Muelleri中必需多不饱和脂肪酸20:5n-3的合成途径。

本研究试图表征产生必需多不饱和脂肪酸(PUFA)20:5n-3(EPA)的合成途径。为此,在硅藻Chaetoceros muelleri生长24小时的过程中,通过实验监测了13 C掺入10种脂肪酸(FA)中的情况Chaetoceros muelleri优先快速地将13 C掺入C 18 PUFA中,例如18:2n-6和18:3n-6以及16:0和16:1n-7,因此非常高1 3 富含C。在实验期间,20:5n-3和16:3n-4是最不富含脂肪酸的脂肪酸。通过计算脂肪酸B与其可疑前体A的富集百分比,我们可以得出结论:硅藻通过n-3(通过18:4n-3)与n-3(与之结合)产生20:5n-3(EPA)。 n-6(通过18:3n-6和20:4n-6)合成途径以及替代的ω-3去饱和酶途径(通过20:4n-6)。此外,由于极性脂质中的FA通常会更富集13种C比FA来自中性脂质,特别是对于18:1n-9、18:2n-6和18:3n-6,还假设存在酰基编辑机制以及极性和中性脂质脂肪酸池之间的连通性。由于16:3n-4和20:5n-3呈现相同的浓度和富集动力学,因此在穆勒梭菌中这两个PUFA之间提出了结构和代谢联系。
更新日期:2020-05-21
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