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In Vitro Anti-Inflammatory, Anti-Oxidant, and Cytotoxic Activities of Four Curcuma Species and the Isolation of Compounds from Curcuma aromatica Rhizome.
Biomolecules ( IF 5.5 ) Pub Date : 2020-05-21 , DOI: 10.3390/biom10050799 Aknarin Pintatum 1 , Wisanu Maneerat 1, 2 , Emilie Logie 3 , Emmy Tuenter 4 , Maria E Sakavitsi 5 , Luc Pieters 4 , Wim Vanden Berghe 3 , Tawanun Sripisut 6 , Suwanna Deachathai 1 , Surat Laphookhieo 1, 2
Biomolecules ( IF 5.5 ) Pub Date : 2020-05-21 , DOI: 10.3390/biom10050799 Aknarin Pintatum 1 , Wisanu Maneerat 1, 2 , Emilie Logie 3 , Emmy Tuenter 4 , Maria E Sakavitsi 5 , Luc Pieters 4 , Wim Vanden Berghe 3 , Tawanun Sripisut 6 , Suwanna Deachathai 1 , Surat Laphookhieo 1, 2
Affiliation
The genus Curcuma is part of the Zingiberaceae family, and many Curcuma species have been used as traditional medicine and cosmetics in Thailand. To find new cosmeceutical ingredients, the in vitro anti-inflammatory, anti-oxidant, and cytotoxic activities of four Curcuma species as well as the isolation of compounds from the most active crude extract (C. aromatica) were investigated. The crude extract of C. aromatica showed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with an IC50 value of 102.3 μg/mL. The cytotoxicity effect of C. aeruginosa, C. comosa, C. aromatica, and C. longa extracts assessed with the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 200 μg/mL were 12.1 2.9, 14.4 4.1, 28.6 4.1, and 46.9 8.6, respectively. C. aeruginosa and C. comosa presented apoptosis cells (57.7 3.1% and 32.6 2.2%, respectively) using the CytoTox-ONE™ assay. Different crude extracts or phytochemicals purified from C. aromatica were evaluated for their anti-inflammatory properties. The crude extract of C. aromatica showed the highest potential to inhibit NF-κB activity, followed by C. aeruginosa, C. comosa, and C. longa, respectively. Among the various purified phytochemicals curcumin, germacrone, curdione, zederone, and curcumenol significantly inhibited NF-κB activation in tumor necrosis factor (TNF) stimulated HaCaT keratinocytes. Of all compounds, curcumin was the most potent anti-inflammatory.
中文翻译:
四种姜黄素的体外抗炎,抗氧化剂和细胞毒活性以及从姜黄根茎中分离化合物的研究。
属莪是姜科的一部分,许多莪物种已被用来作为传统医药和化妆品在泰国。为了找到新的药妆成分,研究了四种姜黄的体外抗炎,抗氧化和细胞毒性活性,以及从活性最强的粗提物(C.aromatica)分离化合物的方法。芳香丙酸梭菌的粗提物显示出2,2-二苯基-1-吡啶并肼基(DPPH)的自由基清除活性,IC 50值为102.3μg/ mL。的细胞毒性作用C.假单胞菌,C. comosa,C.郁金,和C.隆加经3- [4,5-二甲基噻唑-2-基] -2,5-二苯基溴化四唑(MTT)分析评估的提取物分别为12.1 2.9、14.4 4.1、28.6 4.1和46.9 8.6。使用CytoTox-ONE™分析法,铜绿假单胞菌和com。comosa呈现凋亡细胞(分别为57.7 3.1%和32.6 2.2%)。评价了从芳香族假丝酵母纯化的不同粗提取物或植物化学物质的抗炎特性。芳香油茶的粗提物具有最高的抑制NF-κB活性的能力,其次是铜绿油茶,com。comosa和C. longa。, 分别。在各种纯化的植物化学物质中,姜黄素,胚芽酮,姜二酮,Zederone和姜黄烯醇显着抑制肿瘤坏死因子(TNF)刺激的HaCaT角质形成细胞中的NF-κB活化。在所有化合物中,姜黄素是最有效的抗炎药。
更新日期:2020-05-21
中文翻译:
四种姜黄素的体外抗炎,抗氧化剂和细胞毒活性以及从姜黄根茎中分离化合物的研究。
属莪是姜科的一部分,许多莪物种已被用来作为传统医药和化妆品在泰国。为了找到新的药妆成分,研究了四种姜黄的体外抗炎,抗氧化和细胞毒性活性,以及从活性最强的粗提物(C.aromatica)分离化合物的方法。芳香丙酸梭菌的粗提物显示出2,2-二苯基-1-吡啶并肼基(DPPH)的自由基清除活性,IC 50值为102.3μg/ mL。的细胞毒性作用C.假单胞菌,C. comosa,C.郁金,和C.隆加经3- [4,5-二甲基噻唑-2-基] -2,5-二苯基溴化四唑(MTT)分析评估的提取物分别为12.1 2.9、14.4 4.1、28.6 4.1和46.9 8.6。使用CytoTox-ONE™分析法,铜绿假单胞菌和com。comosa呈现凋亡细胞(分别为57.7 3.1%和32.6 2.2%)。评价了从芳香族假丝酵母纯化的不同粗提取物或植物化学物质的抗炎特性。芳香油茶的粗提物具有最高的抑制NF-κB活性的能力,其次是铜绿油茶,com。comosa和C. longa。, 分别。在各种纯化的植物化学物质中,姜黄素,胚芽酮,姜二酮,Zederone和姜黄烯醇显着抑制肿瘤坏死因子(TNF)刺激的HaCaT角质形成细胞中的NF-κB活化。在所有化合物中,姜黄素是最有效的抗炎药。