当前位置: X-MOL 学术Science › 论文详情
Ion transport and regulation in a synaptic vesicle glutamate transporter.
Science ( IF 41.037 ) Pub Date : 2020-05-22 , DOI: 10.1126/science.aba9202
Fei Li,Jacob Eriksen,Janet Finer-Moore,Roger Chang,Phuong Nguyen,Alisa Bowen,Alexander Myasnikov,Zanlin Yu,David Bulkley,Yifan Cheng,Robert H Edwards,Robert M Stroud

Synaptic vesicles accumulate neurotransmitters, enabling the quantal release by exocytosis that underlies synaptic transmission. Specific neurotransmitter transporters are responsible for this activity and therefore are essential for brain function. The vesicular glutamate transporters (VGLUTs) concentrate the principal excitatory neurotransmitter glutamate into synaptic vesicles, driven by membrane potential. However, the mechanism by which they do so remains poorly understood owing to a lack of structural information. We report the cryo-electron microscopy structure of rat VGLUT2 at 3.8-angstrom resolution and propose structure-based mechanisms for substrate recognition and allosteric activation by low pH and chloride. A potential permeation pathway for chloride intersects with the glutamate binding site. These results demonstrate how the activity of VGLUTs can be coordinated with large shifts in proton and chloride concentrations during the synaptic vesicle cycle to ensure normal synaptic transmission.
更新日期:2020-05-21

 

全部期刊列表>>
如何通过Nature平台传播科研成果
跟Nature、Science文章学绘图
隐藏1h前已浏览文章
课题组网站
新版X-MOL期刊搜索和高级搜索功能介绍
中洪博元
ACS材料视界
x-mol收录
南开大学
朱守非
廖良生
南方科技大学
西湖大学
伊利诺伊大学香槟分校
徐明华
中山大学化学工程与技术学院
试剂库存
天合科研
down
wechat
bug