The applicability of ninhydrin, a widely used derivatizing reagent, for determination of teicoplanin (TEIC) in its pure form, pharmaceutical vials, and in human plasma was investigated. The presented spectrofluorimetric method was based on a condensation reaction between ninhydrin and the primary amine group existing in TEIC (in the presence of phenylacetaldehyde) to produce a highly fluorescent product detected at 460 nm (λ_ex,390 nm). Calibration plots were constructed in the concentration range 60–600 ng mL^–1 with a good correlation coefficient of 0.9998 and a low detection limit of 10.84 ng mL^–1. The method was subjected to a bioanalytical validation study according to US‐FDA recommendations. The proposed method was applied for analysis of TEIC in commercial vials with high recovery result 101.88 ± 1.11%. In addition, the method was utilized efficiently for detection of TEIC in human plasma using salting‐out assisted liquid–liquid extraction technique (SALLE) with a recovery range from 96.71 ± 1.08% to 97.71 ± 0.86%. SALLE is an effective approach used for extraction of TEIC from human plasma without interferences using ammonium sulphate. The proposed method is highly recommended to monitor TEIC in clinical laboratory samples and therapeutic drug monitoring systems.

中文翻译：

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茚三酮作为一种荧光试剂在盐析辅助液-液萃取技术中估算替考拉宁在人血浆中的适用性。

研究了广泛使用的衍生化试剂茚三酮在测定纯形式的替考拉宁（TEIC），药物瓶和人体血浆中的适用性。提出的荧光光谱法是基于茚三酮与TEIC中存在的伯胺基团（在苯乙醛存在下）之间的缩合反应，以产生在460 nm（_λex，390 nm）处检测到的高荧光产物。在60–600 ng mL ^–1的浓度范围内构建校正图，具有良好的相关系数0.9998和较低的检出限10.84 ng mL ^–1。该方法根据美国食品和药物管理局的建议进行了生物分析验证研究。该方法用于商业小瓶中的TEIC分析，回收率高101.88±1.11％。此外，该方法通过盐析辅助液液萃取技术（SALLE）被有效地用于检测人血浆中的TEIC，回收率从96.71±1.08％到97.71±0.86％。SALLE是一种有效的方法，可使用硫酸铵从人血浆中提取TEIC，而不会产生干扰。强烈建议使用建议的方法来监测临床实验室样品和治疗药物监测系统中的TEIC。