In this study, we examined the supporting effects of nano-demineralized bone matrix on the cultivation of Wharton's jelly stem cells on acellularized nerve scaffold. Demineralized bone matrix nanoparticles were prepared and characterized by several experiments. Decellularized sciatic nerve scaffolds were prepared and their efficiency was evaluated using histological stainings and biomechanical testing. Results of histological staining indicated that the integrity of the extra cellular matrix components was preserved. Also, the growth and viability of WJSCs on the scaffolds were significantly higher in DBM nanoparticle groups. We conclude that supportive properties of nano-DBM groups showed better cell viability and a suitable microenvironment for proliferation, retention, and adhesion of cells compared with other groups.

在这项研究中，我们研究了纳米脱矿质骨基质对沃顿氏胶冻干细胞在去细胞神经支架上的培养的支持作用。制备了脱矿骨基质纳米颗粒，并通过一些实验对其进行了表征。制备脱细胞的坐骨神经支架，并使用组织学染色和生物力学测试评估其效率。组织学染色结果表明保留了额外的细胞基质成分的完整性。同样，在DBM纳米颗粒组中，WJSCs在支架上的生长和生存力显着更高。我们得出的结论是，与其他组相比，纳米DBM组的支持特性显示出更好的细胞生存力和细胞增殖，保留和粘附的合适微环境。