当前位置: X-MOL 学术Protein Expres. Purif. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Expression, purification and characterization of a cold-adapted dextranase from marine bacteria and its ability to remove dental plaque.
Protein Expression and Purification ( IF 1.6 ) Pub Date : 2020-05-21 , DOI: 10.1016/j.pep.2020.105678
Tian Deng 1 , Yanli Feng 1 , Linxiang Xu 1 , Xiaopeng Tian 1 , Xiaohua Lai 1 , Mingsheng Lyu 2 , Shujun Wang 2
Affiliation  

Dental plaque is a high-incidence health concern, and it is caused by Streptococcus mutans. Dextranase can specifically hydrolyze ɑ-1,6-glycosidic linkages in dextran. It is commonly used in the sugar industry, in the production of plasma substitutes, and the treatment and prevention of dental plaque. In this research work, we successfully cloned and expressed a cold-adapted dextranase from marine bacteria Catenovulum sp. DP03 in Escherichia coli. The recombinant dextranase named Cadex2870 contained a 2511 bp intact open reading frame and encoded 836 amino acids. The expression condition of recombinant strain was 0.1 mM isopropylthio—galactoside (IPTG), and the reduced temperature was 16 °C. The purified enzyme activity was 16.2 U/mg. The optimal temperature and pH of Cadex2870 were 45 °C and pH 8, and it also had catalytic activity at 0 °C. The hydrolysates of Cadex2870 hydrolysis Dextran T70 are maltose, maltotetraose, maltopentose, maltoheptaose and higher molecular weight maltooligosaccharides. Interestingly, 0.5% sodium benzoate, 2% xylitol, 0.5% sodium fluoride, 5% propanediol, 5% glycerin and 2% sorbitol can enhance stability Cadex2870, which are additives in mouthwashes. Additionally, Cadex2870 reduced the formation of dental plaque and effectively degraded formed plaque. Therefore, Cadex2870 shows great promise in commercial applications.



中文翻译:

来自海洋细菌的冷适应葡聚糖酶的表达,纯化和表征及其去除牙菌斑的能力。

牙菌斑是高发病率的健康问题,它是由变形链球菌引起的葡聚糖酶可以特异性地水解葡聚糖中的ɑ-1,6-糖苷键。它通常用于制糖业,血浆替代品的生产以及牙菌斑的治疗和预防。在这项研究工作中,我们成功地从海洋细菌Catenovulum sp。克隆并表达了一种冷适应的葡聚糖酶。DP03在大肠杆菌中。重组葡聚糖酶Cadex2870含有2511 bp完整的开放阅读框,编码836个氨基酸。重组菌株的表达条件为0.1 mM异丙基硫代半乳糖苷(IPTG),降低温度为16°C。纯化的酶活性为16.2U / mg。Cadex2870的最佳温度和pH分别为45°C和pH 8,并且在0°C时也具有催化活性。Cadex2870水解葡聚糖T70的水解产物是麦芽糖,麦芽四糖,麦芽五糖,麦芽七糖和更高分子量的麦芽低聚糖。有趣的是,0.5%的苯甲酸钠,2%的木糖醇,0.5%的氟化钠,5%的丙二醇,5%的甘油和2%的山梨醇可以增强Cadex2870的稳定性,Cadex2870是漱口水中的添加剂。此外,Cadex2870减少了牙菌斑的形成并有效降解了形成的牙菌斑。因此,

更新日期:2020-05-21
down
wechat
bug