This paper introduces the use of microarray data technology with Medicago (Medicago truncatula) microarrays to characterize global changes in the transcript abundance of etiolated Alaska pea (Pisum sativum L.) seedlings grown under microgravity (µg) conditions in comparison with those under artificial 1 g conditions on the International Space Station. Of the 44,000 genes of the Medicago microarray platform, more than 25,000 transcripts of pea seedlings were hybridized, suggesting that the microarray platform for Medicago could be useful in the study of gene expression of etiolated pea seedlings grown under µg conditions in space. Gene array data were analyzed according to stringent criteria that restricted the scored genes for specific hybridization values at least twofold. Expression of 1362 and 1558 genes in proximal side (the proximal side) and distal side of the epicotyl to the cotyledons (the distal side), respectively, were highly affected by µg conditions in space. Of the genes analyzed, 407 of 1362 transcripts in the proximal side and 740 of 1558 transcripts in the distal side were expressed at ratios at least twofold. However, in the presence of the auxin transport inhibitor TIBA, 212 of 399 transcripts and 255 of 477 transcripts were expressed at ratios at least twofold as high in the proximal and the distal sides of epicotyls in the seedlings grown under µg conditions, respectively. Based on Venn diagram analysis, 31 transcripts and 24 transcripts were found to commonly increase and decrease, respectively, under µg conditions in space. Venn analysis revealed six auxin-related genes and three water channel AQUAPORIN genes that were responsive to gravity. Among 6 auxin-related genes, the accumulation of transcripts of Auxin-induced protein 5NG4 and Indole-3-acetic acid-amido synthetase GH3.3 tended to increase, and that of Auxin-induced protein, Auxin response factor, SAUR-like auxin-responsive family protein and Auxin response factor tended to decrease under µg conditions, whereas there were no statistic differences between under µg and artificial 1 g conditions. Similarly there were no statistic differences between under µg conditions and artificial 1 g, but the accumulation of NIP3-1 and Plasma membrane intrinsic protein11, and AQUAPORIN1/Tonoplast intrinsic protein tended to increase and decrease, respectively. A possible role of auxin-related genes and AQUAPORIN genes in regulating growth of etiolated pea seedlings grown under µg conditions in space is discussed.

本文介绍了将微阵列数据技术与Medicago（Medicago truncatula）微阵列一起用于表征微重力（µ g）条件下与人工条件下相比，在微重力条件下 生长的黄化阿拉斯加豌豆（Pisum sativum L.）幼苗转录本丰度的总体变化。g国际空间站上的条件。的44000个基因的微阵列苜蓿平台的，豌豆幼苗的超过25,000的转录物进行杂交，这表明对于苜蓿微阵列平台可以是下μ生长黄化豌豆幼苗的基因表达的研究有用克空间条件。根据严格的标准分析基因阵列数据，所述严格的标准将评分的基因限制为特定杂交值至少两倍。1 g和1558基因在子叶的上胚轴的近侧（近侧）和远侧（远侧）的表达分别受到空间中微克条件的高度影响。在所分析的基因中，近端1362个转录物中的407个和远端1558个转录物中的740个以至少两倍的比率表达。然而，在植物生长素输送抑制剂TIBA的存在下，399个转录物212和477倍的转录物255物在比表示至少两倍一样高的近端和在下μ生长幼苗上胚轴的远端侧克条件。根据维恩图分析，发现在空间中的微克条件下，分别有31个转录本和24个转录本共同增加和减少。Venn分析揭示了六个对生长素相关的基因和三个水通道AQUAPORIN基因，它们对重力有反应。在生长素相关基因的6个基因中，生长素诱导蛋白5NG4和吲哚-3-乙酸-酰胺基合成酶GH3.3的转录本积累有增加的趋势，而生长素诱导蛋白，生长素反应因子，SAUR样生长素的转录本则有积累。响应性家族蛋白和生长素响应因子趋于下μ减小克在微克和人工1 克条件下没有统计学差异。同样，在μg条件下和人工1 g条件 下也没有统计学差异，但是NIP3-1和质膜内在蛋白11以及AQUAPORIN1 / Tonoplast内在蛋白的积累分别倾向于增加和减少。讨论了生长素相关基因和AQUAPORIN基因在调节微克空间条件下生长的黄化豌豆幼苗生长中的可能作用。