Background

A combination of 0.25 mg/ml green tea (leaves of Camellia sinensis var. assamica) and 7.82 mg/ml Salvadora persica L. root-sticks aqueous extract mouthwash (CoM) has been reported to exhibit antibacterial and anti-adherence effects against primary plaque colonizer biofilm in vitro and an antiplaque effect for a period of 24 h in vivo.

Aim

To assess the antiplaque effect of CoM during a four-day period.

Methods

A four-day plaque re-growth randomized crossover trial was conducted. A week after receiving dental scaling and polishing, all participants (n = 15) underwent polishing at baseline and a rinse with 15 ml of randomly allocated mouthwash (CoM, 0.12 % chlorhexidine (CHX) mouthwash, or placebo [Plc]) twice daily without oral hygiene measures for four days. After 2 h, samples of saliva were collected, using a quantitative polymerase chain reaction, to determine the bacterial load. After 24 h and four days’ time points, plaque quantity (using digital plaque image analysis) was recorded. Subsequently, the participants entered a six-day washout period with regular oral hygiene measures. The same protocol was repeated for the next two mouthwashes.

Results

CoM significantly reduced plaque accumulation (31.933 ± 10.025) compared to Plc (54.629 ± 17.555) for a period of four days; a comparable effect was ascribed to CHX (34.903 ± 11.871). Moreover, it significantly reduced the amount of Streptococcus sanguinis, Actinomyces viscosus, and Actinomyces naeslundii (primary colonizers) in saliva.

Conclusion

Using CoM for four days twice daily could reduce plaque accumulation and might be considered as an alternative to synthetic mouthwashes.

中文翻译：

---

为期四天的随机对照试验，评估了绿茶和Salvadora persica L.漱口水组合的抗牙菌斑作用

背景

的0.25mg / ml的绿茶的组合（叶茶树变种普洱茶）和7.82毫克/毫升萨尔瓦多桃L.根枝水提取物的漱口水（COM）已经报道了表现出对主斑块抗菌剂和抗粘附作用在体外定植生物膜，并在体内24小时内具有抗菌斑作用。

目标

评估四天内CoM的抗斑作用。

方法

进行了为期四天的斑块重新生长随机交叉试验。接受牙齿除垢和抛光一周后，所有参与者（n = 15）在基线进行抛光，并用15 ml随机分配的漱口水（CoM，0.12％洗必泰（CHX）漱口水或安慰剂[Plc]）冲洗两次，每天两次持续四天的口腔卫生措施。2小时后，使用定量聚合酶链反应收集唾液样本，以确定细菌载量。在24小时和四天的时间点之后，记录菌斑量（使用数字菌斑图像分析）。随后，参加者进入为期六天的冲洗期，并定期进行口腔卫生措施。接下来的两次漱口水重复相同的步骤。

结果

与Plc（54.629±17.555）相比，CoM在4天期间显着减少了斑块积聚（31.933±10.025）。CHX（34.903±11.871）产生了可比的效果。此外，它显着减少了唾液中的血链球菌，粘性放线菌和纳氏放线菌（主要定居者）的数量。

结论

每天两次，每天两次使用CoM可以减少牙菌斑的积聚，并且可以被视为合成漱口水的替代品。