Chitosan Nanoparticles Eugenol recognizes as a potent antioxidant that can use the first therapeutic chemical to treat rheumatoid arthritis (RA) instead of Methotrexate. The purpose of this study was to investigate the effects of Chitosan Nanoparticles Eugenol as a potent Nano-herbal agent in the healing process of experimental neonatal RA compared to Methotrexate.

The neonatal Wistar rats induced rheumatoid arthritis in both genders were divided into sham, control, the treatment receiving Methotrexate, and the second treatment receiving encapsulated Eugenol by Chitosan Nanoparticles groups. Afterward, Malondialdehyde, for assessment of lipid peroxidation as an oxidative stress biomarker by assay kit, FOXO3 protein as an antioxidant up-regulating by western blotting and expression of the TGF-β and CCL2/MCP-1 genes by real-time PCR evaluation, supported by a cartilage histopathology analysis. Based on these results, Methotrexate and Eugenol encapsulated by Chitosan Nanoparticles, a significant decrease is observed in the serum level of MDA and FOXO3 protein expression in comparison to the control group. Additionally, Nanoparticle herbal agent and Methotrexate has a decreasing effect on the expression of TGF-β and MCP-1 genes and a significant positive correlation was observed between MCP-1 and TGF-β. Inflammation, synovial hyperplasia, and pannus formation were extreme in the Collagen Induced Arthritis rats. It can be concluded that Encapsulated Eugenol by Chitosan Nanoparticles and Methotrexate, probably by dint of their immunomodulatory, anti-inflammatory, and antioxidant potential has a protective effect against RA. Nano Eugenol is capable of delivering promising lines results to treat autoimmune diseases such as RA can also be suggested.

壳聚糖纳米粒丁香酚被公认为是有效的抗氧化剂，可以使用第一种治疗性药物代替类甲氨蝶呤治疗类风湿性关节炎（RA）。这项研究的目的是研究与甲氨蝶呤相比，壳聚糖纳米粒丁香酚作为强效纳米草药制剂在实验性新生儿RA愈合过程中的作用。

新生的Wistar大鼠将两种性别诱导的类风湿性关节炎分为假手术，对照组，接受甲氨蝶呤的治疗和接受壳聚糖纳米粒子治疗的第二种接受包封的丁香酚的治疗。之后，丙二醛通过分析试剂盒评估脂质过氧化作为氧化应激生物标记物，FOXO3蛋白作为抗氧化剂通过Western印迹上调，并通过实时PCR评估TGF-β和CCL2 / MCP-1基因的表达，由软骨组织病理学分析支持。基于这些结果，甲氨蝶呤和丁子香酚包封由壳聚糖纳米颗粒，一个显著减少是在MDA和FOXO3蛋白表达的血清水平观察到相比于对照组。此外，纳米草药制剂和甲氨蝶呤š对TGF-β和MCP-1基因和MCP-1和TGF-β之间观察到显著正相关的表达的降低效果。在胶原诱导的关节炎大鼠中，炎症，滑膜增生和pan的形成极度严重。由此可以得出结论，封装丁香酚由壳聚糖纳米颗粒和氨甲喋呤，可能是由它们的免疫调节力，抗炎，抗氧化和潜在公顷š对RA有保护作用。纳米丁香酚能够提供有前途的结果来治疗自身免疫性疾病，如RA。