Background

Chemokine-like factor 1 (CKLF1) is a chemokine increased significantly in ischemic brain poststroke. It shows chemotaxis effects on various immune cells, but the mechanisms of CKLF1 migrating neutrophils are poorly understood. Recent studies have provided evidence that CC chemokine receptor 5 (CCR5), a receptor of CKLF1, is involved in ischemic stroke.

Purposes

To investigate the effects of HIF-1α guided AAV in ischemic brain, investigating the outcome of stroke, and examining the involvement of CKLF1/CCR5 axis in recruitment of neutrophils.

Results

HIF-1α guided AAV knocked down CKLF1 in ischemic area and alleviated brain damage of rats. CKLF1 migrated neutrophils through CCR5, worsening inflammatory responses. Akt/GSK-3β pathway may involve in CKLF1/CCR5 axis guided neutrophils chemotaxis.

Conclusions

CKLF1/CCR5 axis is involved in neutrophils migration of rats with transient cerebral ischemia. CKLF1/CCR5 axis may be a useful target for stroke therapy.

中文翻译：

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CKLF1 / CCR5轴参与短暂性脑缺血大鼠的中性粒细胞迁移。

背景

趋化因子样因子1（CKLF1）是缺血性脑卒中后明显增加的趋化因子。它显示出对各种免疫细胞的趋化作用，但对CKLF1迁移中性粒细胞的机制了解甚少。最近的研究提供了证据，即CKLF1受体CC趋化因子受体5（CCR5）与缺血性中风有关。

目的

为了研究HIF-1α引导的AAV在缺血性脑中的作用，研究中风的结果，并检查CKLF1 / CCR5轴参与中性粒细胞的募集。

结果

HIF-1α指导的AAV在缺血区域敲低CKLF1，减轻大鼠脑损伤。CKLF1通过CCR5迁移嗜中性粒细胞，加剧了炎症反应。Akt /GSK-3β途径可能参与CKLF1 / CCR5轴引导的嗜中性粒细胞趋化性。

结论

CKLF1 / CCR5轴参与短暂性脑缺血大鼠的中性粒细胞迁移。CKLF1 / CCR5轴可能是中风治疗的有用靶标。