The antidepressant activities of hispidol and decursin (both potent monoamine oxidase A (MAO-A) inhibitors) were evaluated using the forced swimming test (FST) and the tail suspension test (TST) in mice, and thereafter, levels of neurotransmitter monoamines and metabolites in brain tissues were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Hispidol (15 mg/kg) caused less or comparable immobility than fluoxetine (15 mg/kg; the positive control) in immobility time, as determined by FST (9.6 vs 32.0 s) and TST (53.1 vs 48.7 s), respectively, and its effects were dose-dependent and significant. Decursin (15 mg/kg) also produced immobility comparable to that of fluoxetine as determined by FST (47.0 vs 43.4 s) and TST (55.6 vs 63.4 s), and its effects were also dose-dependent and significant. LC-MS/MS analysis after FST showed that hispidol (15 mg/kg) greatly increased dopamine (DA) and serotonin levels dose-dependently in brain tissues as compared with the positive control. Decursin (15 mg/kg) dose-dependently increased DA level after TST. Slight changes in norepinephrine and 3,4-dihydroxyphenylacetic acid levels were observed after FST and TST in hispidol- or decursin-treated animals. It was observed that hispidol and decursin were effective and comparable to fluoxetine in immobility tests. These immobility and monoamine level results suggest that hispidol and decursin are potential antidepressant agents for the treatment of depression, and that they act mainly through serotonergic and/or dopaminergic systems.

中文翻译：

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Hispidol和Decursin在小鼠中的抗抑郁样活性以及神经递质单胺的分析。

使用强迫游泳试验（FST）和尾部悬吊试验（TST）评估了小鼠组氨酸和递精蛋白（两种有效的单胺氧化酶A（MAO-A）抑制剂）的抗抑郁活性，然后评估了神经递质单胺和代谢物的水平用液相色谱-串联质谱法（LC-MS / MS）分析脑组织中的蛋白。由FST（9.6 vs 32.0 s）和TST（53.1 vs 48.7 s）分别确定，Hispidol（15 mg / kg）导致的固定时间比氟西汀（15 mg / kg；阳性对照）少或类似。其作用是剂量依赖性和显着的。通过FST（47.0 vs 43.4 s）和TST（55.6 vs 63.4 s）测定，地精（15 mg / kg）也产生了与氟西汀相当的固定性，并且其作用也呈剂量依赖性和显着性。FST后的LC-MS / MS分析表明，与阳性对照相比，组胺（15 mg / kg）剂量依赖性地大大增加了脑组织中的多巴胺（DA）和血清素水平。TST后地精素（15 mg / kg）剂量依赖性地增加DA水平。FST和TST后，在经组氨醇或去壁素处理的动物中观察到去甲肾上腺素和3,4-二羟基苯基乙酸的水平有轻微变化。观察到在固定性测试中，组氨醇和去壁素是有效的，并且与氟西汀相当。这些固定性和单胺水平的结果表明，组氨醇和递精蛋白是用于治疗抑郁症的潜在抗抑郁药，并且它们主要通过血清素能和/或多巴胺能系统起作用。