Gibberellins (GAs) are a group of plant hormones that play important roles in various processes. Previous studies demonstrated that GA can increase the cellulose content and lignification of plant tissues. However, the effects of altered GA levels on carrot root development have not been fully characterized. In this study, carrot roots were treated with exogenous GA 3 , after which thickened cell walls were detected in the xylem area. Transcriptome sequencing data revealed the changes induced by GA 3 . A total of 5416 differentially expressed genes (DEGs) were identified, of which 3011 and 2405 were up- and down-regulated, respectively. The DEGs were enriched in metabolic pathways, with 236 DEGs related to the cell wall. Digital gene expression data and the verification by qRT-PCR indicated that exogenous GA can alter the expression of genes involved in cell wall synthesis, suggesting a potential role for GA in cell wall organization and modification. The results of this study provide novel insights into the GA functions influencing carrot root development.

中文翻译：

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赤霉素诱导胡萝卜根木质部细胞壁相关基因表达的改变

赤霉素 (GA) 是一组在各种过程中发挥重要作用的植物激素。先前的研究表明，GA 可以增加植物组织的纤维素含量和木质化。然而，改变 GA 水平对胡萝卜根发育的影响尚未完全表征。在这项研究中，胡萝卜根用外源 GA 3 处理，之后在木质部区域检测到增厚的细胞壁。转录组测序数据揭示了 GA 3 引起的变化。共鉴定出 5416 个差异表达基因 (DEG)，其中分别上调和下调了 3011 个和 2405 个。DEG 富含代谢途径，其中 236 个 DEG 与细胞壁相关。数字基因表达数据和 qRT-PCR 验证表明，外源 GA 可以改变参与细胞壁合成的基因的表达，表明 GA 在细胞壁组织和修饰中具有潜在作用。这项研究的结果为影响胡萝卜根发育的 GA 功能提供了新的见解。