This article reports a simple, convenient, and very sensitive method for the determination of 6-thio guanine (6-TG). The basic phenomenon in the proposed method is fluorescence resonance energy transfer, where acridine orange (AO) act as a donor and citrate-stabilized silver nanoparticles (AgNPs) as an acceptor. A noncovalent bond between the surfaces of AgNPs and AO was found, and the effect of fluorescence quenching was observed. The fluorescence spectra of AO recovered after further addition of 6-TG are responsible for the aggregation of AgNPs. Under ideal conditions, the linear relationship of 6-TG in the concentration range of 0.005 to 0.04 μM was displayed, and the limit of detection of 6-TG was obtained as 5.3 nM. Under ideal conditions, the linear relationship of 6-TG was displayed in the concentration range of 0.005 to 0.04 μM, and the limit of detection of 6-TG was obtained as 5.3 nM. The proposed method offers a rapid analysis to determine 6-TG in human serum, blood, and urine samples.

中文翻译：

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基于银纳米粒子介导的particle啶橙探针的释放超灵敏荧光测定生物样品中的6-硫鸟嘌呤

本文报道了一种简单，方便且非常灵敏的测定6-硫代鸟嘌呤（6-TG）的方法。提出的方法中的基本现象是荧光共振能量转移，其中（啶橙（AO）充当供体，柠檬酸盐稳定的银纳米颗粒（AgNPs）充当受体。发现AgNPs和AO表面之间的非共价键，并观察到荧光猝灭的效果。进一步添加6-TG后回收的AO的荧光光谱负责AgNP的聚集。在理想条件下，显示了0.005-0.04μM浓度范围内6-TG的线性关系，并且6-TG的检出限为5.3 nM。在理想条件下，6-TG的线性关系在0.005至0.04μM的浓度范围内显示，6-TG的检出限为5.3 nM。拟议的方法提供了一种快速分析方法，用于测定人血清，血液和尿液样品中的6-TG。