A computationally predicted gene regulatory network (GRN), generated from mantle-specific gene expression profiles in the Antarctic clam Laternula elliptica, was interrogated to test the regulation and interaction of duplicated inducible hsp70 paralogues. hsp70A and hsp70B were identified in the GRN with each paralogue falling into unique submodules that were linked together by a single shared second neighbour. Annotations associated with the clusters in each submodule suggested that hsp70A primarily shares regulatory relationships with genes encoding ribosomal proteins, where it may have a role in protecting the ribosome under stress. hsp70B, on the other hand, interacted with a suite of genes involved in signalling pathways, including four transcription factors, cellular response to stress and the cytoskeleton. Given the contrasting submodules and associated annotations of the two hsp70 paralogues, the GRN analysis suggests that each gene is carrying out additional separate functions, as well as being involved in the traditional chaperone heat stress response, and therefore supports the hypothesis that subfunctionalization has occurred after gene duplication. The GRN was specifically produced from experiments investigating biomineralization; however, this study shows the utility of such data for investigating multiple questions concerning gene duplications, interactions and putative functions in a non-model species.

中文翻译：

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基因网络分析支持南极蛤中重复 hsp70 基因的亚功能化假设。

一个计算预测的基因调控网络 (GRN)，从南极蛤 Latenula elliptica 的地幔特异性基因表达谱中生成，被询问以测试重复诱导型 hsp70 旁系同源物的调控和相互作用。hsp70A 和 hsp70B 在 GRN 中被识别，每个旁系同源物都属于独特的子模块，这些子模块由一个共享的第二个邻居连接在一起。与每个子模块中的簇相关的注释表明 hsp70A 主要与编码核糖体蛋白的基因共享调节关系，它可能在压力下保护核糖体。另一方面，hsp70B 与一组参与信号通路的基因相互作用，包括四种转录因子、细胞对压力的反应和细胞骨架。鉴于两个 hsp70 paralogues 的对比子模块和相关注释，GRN 分析表明每个基因都在执行额外的独立功能，并参与传统的伴侣热应激反应，因此支持亚功能化发生在基因重复。GRN 是专门从研究生物矿化的实验中产生的；然而，这项研究显示了此类数据可用于研究有关非模式物种中基因重复、相互作用和假定功能的多个问题。因此支持基因复制后发生亚功能化的假设。GRN 是专门从研究生物矿化的实验中产生的；然而，这项研究显示了此类数据可用于研究有关非模式物种中基因重复、相互作用和假定功能的多个问题。因此支持基因复制后发生亚功能化的假设。GRN 是专门从研究生物矿化的实验中产生的；然而，这项研究显示了此类数据可用于研究有关非模式物种中基因重复、相互作用和假定功能的多个问题。