During sexual reproduction, development of a totipotent zygote from the fusion of highly differentiated gametes is accompanied by dynamic regulation of gene expression. This notably involves RNA silencing operated by Argonautes (AGO) effector proteins. While AGOs roles during Arabidopsis somatic life have been extensively investigated, less is known about their expression during reproduction, which proceeds via double-fertilization of an egg and a central cell, leading respectively to the embryo and a supportive tissue known as endosperm. Using full-locus translational reporters for all ten Arabidopsis AGOs, we uncover cell-specific expression patterns and AGO-intrinsic subcellular localizations in reproductive tissues. However, while some Arabidopsis AGOs are enriched in both male and female gametes, i.e. sperm and egg cells, they are comparably low-expressed in accessory, i.e. vegetative and central cells. Likewise, following fertilization, several AGOs are expressed in the early embryo, yet below detection in the early endosperm. Thus, there is pre- and post-fertilization asymmetry between the embryo and endosperm lineages. Later during embryo development, AGO9, AGO5 and AGO7 are restricted to the apical embryonic meristem in contrast to AGO1, AGO4, AGO6 and AGO10. Beside shedding light onto potential roles for RNA silencing during Arabidopsis reproduction, the plant material generated here should constitute a valuable asset enabling functional AGOs studies in many tissues beyond those involved in reproduction.

中文翻译：

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拟南芥生殖组织中非典型表达

在有性生殖期间，由高度分化的配子融合而形成的全能合子伴随着基因表达的动态调节。这尤其涉及由Argonautes（AGO）效应子蛋白操作的RNA沉默。尽管已经对AGO在拟南芥体细胞生活中的作用进行了广泛研究，但对它们在生殖过程中的表达知之甚少，这种表达是通过卵和中央细胞的双重受精而进行的，分别导致胚胎和被称为胚乳的支持组织。使用所有十个拟南芥AGO的全位翻译记者，我们发现生殖组织中的细胞特异性表达模式和AGO内在亚细胞定位。但是，尽管一些拟南芥AGO富含男性和女性配子，即精子和卵细胞，它们在辅助细胞（即营养细胞和中枢细胞）中的表达相对较低。同样，受精后，一些AGO在早期胚胎中表达，但低于早期胚乳中的表达。因此，在胚胎和胚乳谱系之间存在受精前后的不对称性。在胚胎发育的后期，与AGO1，AGO4，AGO6和AGO10相比，AGO9，AGO5和AGO7仅限于顶端的胚胎分生组织。除了阐明拟南芥繁殖过程中RNA沉默的潜在作用外，此处产生的植物材料应构成宝贵的资产，使功能性AGOs能够在涉及繁殖的组织之外的许多组织中进行研究。仍未在早期胚乳中发现。因此，在胚胎和胚乳谱系之间存在受精前后的不对称性。在胚胎发育的后期，与AGO1，AGO4，AGO6和AGO10相比，AGO9，AGO5和AGO7仅限于顶端的胚胎分生组织。除了阐明拟南芥繁殖过程中RNA沉默的潜在作用外，此处产生的植物材料应构成宝贵的资产，使AGOs能够在涉及繁殖的组织之外的许多组织中进行功能性研究。仍未在早期胚乳中发现。因此，在胚胎和胚乳谱系之间存在受精前后的不对称性。在胚胎发育的后期，与AGO1，AGO4，AGO6和AGO10相比，AGO9，AGO5和AGO7仅限于顶端的胚胎分生组织。除了阐明拟南芥繁殖过程中RNA沉默的潜在作用外，此处产生的植物材料应构成宝贵的资产，使AGOs能够在涉及生殖的组织之外的许多组织中进行功能性研究。