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Viral loads in throat and anal swabs in children infected with SARS-CoV-2.
Emerging Microbes & Infections ( IF 13.2 ) Pub Date : 2020-06-09 , DOI: 10.1080/22221751.2020.1771219
Chunhui Yuan 1 , Hongmin Zhu 2 , Yuan Yang 3 , Xiaonan Cai 3 , Feiyan Xiang 3 , Huan Wu 1 , Cong Yao 4 , Yun Xiang 1 , Han Xiao 3
Affiliation  

ABSTRACT

Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performance of RT-PCR assay on throat and anal swabs remain limited in paediatric patients. Here, we retrospectively reviewed RT-PCR-testing results of 212 paediatric patients with suspected SARS-CoV-2 infection at Wuhan Children’s Hospital. The diagnostic potential of these two types of specimens showed significant difference (positive rate: 78.2% on throat swabs vs. 52.6% on anal swabs, McNemar Test P = 0.0091) and exhibited a weak positive consistency (Kappa value was 0.311, P < 0.0001) in paediatric patients. Furthermore, viral loads detected on both throat and anal swabs also showed no significant difference (P = 0.9511) and correlation (Pearson r = 0.0434, P = 0.8406), and exhibited an inconsistent kinetic change through the course of SARS-CoV-2 infection. Besides, viral loads in the throat and anal swabs were correlated with different types of immune states, immune-reactive phase, and the resolution phase/immunologic tolerance, respectively. These findings revealed that RT-PCR-testing on throat and anal swabs showed significant difference for monitoring SARS-CoV-2 infection and correlated with different immune state in paediatric patients.



中文翻译:

感染SARS-CoV-2的儿童的喉咙和肛门拭子中的病毒载量。

摘要

据报道,即使在严重急性呼吸系统综合症冠状病毒2(SARS-CoV-2)感染中,即使喉咙检测为阴性,对拭子的实时逆转录聚合酶链反应(RT-PCR)测定仍是持续阳性。但是,在儿科患者中,有关咽喉和肛门拭子上RT-PCR测定的一致性能的数据仍然有限。在这里,我们回顾性分析了武汉市儿童医院212例疑似SARS-CoV-2感染的儿科患者的RT-PCR检测结果。这两类标本的诊断潜力显示出显着差异(阳性率:咽拭子为78.2%,而肛门拭子为52.6%,McNemar测试P  = 0.0091)并且呈弱阳性一致性(Kappa值为0.311,P <0.0001)在儿科患者中。此外,在咽喉和肛门拭子上检测到的病毒载量也没有显示出显着差异(P  = 0.9511)和相关性(Pearson r  = 0.0434,P  = 0.8406),并且在SARS-CoV-2感染过程中显示出不一致的动力学变化。 。此外,喉咙和肛门拭子中的病毒载量分别与不同类型的免疫状态,免疫反应阶段和消退阶段/免疫耐受性相关。这些发现表明,对儿科患者的咽喉和肛门拭子进行RT-PCR检测显示出监测SARS-CoV-2感染的显着差异,并与不同的免疫状态相关。

更新日期:2020-06-09
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