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Platycodin D inhibits MPP+-induced inflammatory response in BV-2 cells through the TLR4/MyD88/NF-κB signaling pathway
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-05-19 , DOI: 10.1080/10799893.2020.1767135
Fu Sun 1 , Fengguo Liu 1
Affiliation  

Abstract Parkinson’s disease is a common progressive neurodegenerative disorder associated with inflammation. Platycodin D (PLD) is a triterpenesaponin that has anti-inflammatory and neuro-protective effects. However, the role of PLD in Parkinson’s disease has not been fully investigated. In the current study, we investigated the effect of PLD on 1-methyl-4-phenylpyridinium (MPP+)-induced inflammatory response in BV-2 cells. Our results showed that PLD treatment improved the cell viability of MPP+-induced BV-2 cells. PLD significantly inhibited the levels of inflammatory mediators including nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in MPP+-treated BV-2 cells. The increased productions of inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and IL-6 in MPP+-treated BV-2 cells were also suppressed by PLD. Furthermore, PLD inhibited the activation of TLR4/MyD88/NF-κB pathway in MPP+-treated BV-2 cells. Overexpression of TLR4 reversed the protective effects of PLD on MPP+-treated BV-2 cells. Collectively, PLD protected BV-2 cells from MPP+-induced inflammatory response via regulating the TLR4–MyD88–NF-κB signaling pathway.

中文翻译:

桔梗素 D 通过 TLR4/MyD88/NF-κB 信号通路抑制 MPP+ 诱导的 BV-2 细胞炎症反应

摘要 帕金森病是一种常见的与炎症相关的进行性神经退行性疾病。桔梗素 D (PLD) 是一种三萜皂苷,具有抗炎和神经保护作用。然而,PLD 在帕金森病中的作用尚未得到充分研究。在当前的研究中,我们研究了 PLD 对 BV-2 细胞中 1-甲基-4-苯基吡啶 (MPP+) 诱导的炎症反应的影响。我们的结果表明,PLD 处理提高了 MPP+ 诱导的 BV-2 细胞的细胞活力。PLD 显着抑制 MPP+ 处理的 BV-2 细胞中炎症介质的水平,包括一氧化氮 (NO)、前列腺素 E2 (PGE2)、诱导型一氧化氮合酶 (iNOS) 和环氧合酶-2 (COX-2)。炎性细胞因子肿瘤坏死因子-α(TNF-α)、白介素 1β(IL-1β)、MPP+ 处理的 BV-2 细胞中的 IL-6 和 IL-6 也被 PLD 抑制。此外,PLD 抑制 MPP+ 处理的 BV-2 细胞中 TLR4/MyD88/NF-κB 通路的激活。TLR4 的过表达逆转了 PLD 对 MPP+ 处理的 BV-2 细胞的保护作用。总的来说,PLD 通过调节 TLR4–MyD88–NF-κB 信号通路保护 BV-2 细胞免受 MPP+ 诱导的炎症反应。
更新日期:2020-05-19
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