This investigation describes the formation of crystalline nanotubes of titanium oxide on the surface of a Ti-6Al-4V alloy and its biological evaluation. The formation of nanotubes was performed by the anodic oxidation technique with a constant work potential of 60 V but with different anodizing times of 10, 20, 30, 40, 50, and 60 min used to evaluate their effects on the characteristics of the nanotubes and their biological activity. A mixture of ethylene glycol, water, and ammonium fluoride (NH₄F) was used as the electrolytic fluid. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were applied to determine the morphology and crystalline nature of the nanotubes, showing a well-defined matrix of nanotubes of titanium oxide with a crystalline structure and a diameter in the range of 52.5 ± 5.13 to 95 ± 11.92 nm. In contrast, the XRD patterns showed an increase of defined peaks that directly correlated with treatment times. Moreover, in vitro assays using an innovative cell culture device demonstrated that the inner diameter of the nanotubes directly correlated with cell proliferation.

中文翻译：

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阳极氧化对Ti-6Al-4V合金的表面改性及其对骨关节细胞增殖的影响

这项研究描述了在Ti-6Al-4V合金表面上形成的氧化钛晶体纳米管及其生物学评估。纳米管的形成是通过阳极氧化技术进行的，恒定工作电位为60 V，但阳极氧化时间分别为10、20、30、40、50和60分钟，以评估其对纳米管特性的影响。它们的生物活性。乙二醇，水和氟化铵（NH _4的混合物F）用作电解液。运用扫描电子显微镜（SEM）和X射线衍射（XRD）来确定纳米管的形态和晶体性质，显示出明确定义的具有晶体结构且直径在52.5范围内的氧化钛纳米管基质。 ±5.13至95±11.92 nm 相反，XRD图谱显示了与治疗时间直接相关的确定峰的增加。此外，使用创新的细胞培养装置进行的体外分析表明，纳米管的内径与细胞增殖直接相关。