A novel leaf fall disease of rubber trees (Hevea brasiliensis) has been recently noted in Thailand. The fungal pathogens of this disease were identified based on both morphological and molecular characteristics as Neopestalotiopsis cubana and N. formicarum. Portions of internal transcribed spacer (ITS) region and the large subunit (LSU), translation elongation factor 1‐α (TEF1‐α) and β‐tubulin (TUB) genes were PCR amplified with the primer pairs ITS1/ITS4, LR0R/LR5, EF1‐728F/EF2 and T1/Bt2b, respectively. Sequencing of the PCR products and a phylogenetic tree based on the combined ITS, TEF1‐α and TUB confirmed these pathogens as N. cubana and N. formicarum. Pathogenicity test results showed that the pathogens cause leaf spot and leaf fall similar to that observed in natural infections. This is the first report on the novel leaf fall disease of rubber trees in Thailand, with the results demonstrating that it is associated with N. cubana and N. formicarum.

中文翻译：

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与泰国橡胶树（Hevea brasiliensis）的一种新型叶片倒落病相关的新拟青霉真菌的鉴定和表征

泰国最近发现了一种新型的橡胶树（巴西橡胶树）的落叶病。这种疾病的真菌病原体同时基于形态学和分子特性进行鉴定Neopestalotiopsis古巴和Ñ。福米卡伦。用引物对ITS1 / ITS4，LR0R / LR5进行PCR扩增内部转录间隔区（ITS）和大亚基（LSU），翻译延伸因子1-α（TEF1-α）和β-微管蛋白（TUB）基因的部分，EF1-728F / EF2和T1 / Bt2b。基于ITS，TEF1-α和TUB结合的PCR产物测序和系统发育树证实这些病原体为古巴猪笼草和福美猪笼草。致病性测试结果表明，病原体引起的叶斑和叶落与自然感染中观察到的相似。这是泰国关于橡胶树的新型落叶病的首次报道，结果表明该病与古巴猪笼草和福克猪笼草有关。