Lignocellulosic biomass shows high potential as a renewable feedstock for use in biodiesel production via microbial fermentation. Yarrowia lipolytica, an emerging oleaginous yeast, has been engineered to efficiently convert xylose, the second most abundant sugar in lignocellulosic biomass, into lipids for lignocellulosic biodiesel production. Yet, the lipid yield from xylose or lignocellulosic biomass remains far lower than that from glucose. Here we developed an efficient xylose‐utilizing Y. lipolytica strain, expressing an isomerase‐based pathway, to achieve high‐yield lipid production from lignocellulosic biomass. The newly developed xylose‐utilizing Y. lipolytica, YSXID, produced 12.01 g/L lipids with a maximum yield of 0.16 g/g, the highest ever reported, from lignocellulosic hydrolysates. Consequently, this study shows the potential of isomerase‐based xylose‐utilizing Y. lipolytica for economical and sustainable production of biodiesel and oleochemicals from lignocellulosic biomass.

中文翻译：

---

利用木糖利用解脂耶氏酵母，从木质纤维素生物质中高产脂质

木质纤维素生物质作为通过微生物发酵生产生物柴油的可再生原料具有很高的潜力。解脂耶氏酵母（Yarrowia lipolytica）是一种新兴的含油酵母，经过工程改造，可以有效地将木糖（木质纤维素生物质中第二大丰富的糖）转化为脂质，以生产木质纤维素生物柴油。然而，来自木糖或木质纤维素生物质的脂质产量仍然远低于来自葡萄糖的脂质产量。在这里，我们开发了一种高效的利用木糖的解脂耶氏酵母菌株，表达了一种基于异构酶的途径，以实现木质纤维素生物质的高产脂质生产。新开发的利用木糖的解脂耶氏酵母YSXID从木质纤维素水解物中生产出12.01 g / L脂质，最大产量为0.16 g / g，这是有史以来最高的。因此，本研究显示了利用异构酶的木糖利用解脂耶氏酵母在木质纤维素生物质中经济，可持续地生产生物柴油和油脂化学产品的潜力。