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Salinization and wastewater effects on the growth and some cell contents of Scenedesmus bijugatus.
Saudi Journal of Biological Sciences ( IF 4.4 ) Pub Date : 2020-05-20 , DOI: 10.1016/j.sjbs.2020.05.021
Nouf Mohammed Al-Enazi 1
Affiliation  

The aim of this study is to determine the effect of salinization and wastewater stresses on the growth, some cellular contents (total soluble proteins, total soluble carbohydrates, nucleic acids, and amino acids composition) and ultrastructure using TEM of unicellular green alga Scenedesmus bijugatus. Treatment of S. bijugatus by NaCl at 10 and 50 mg L−1 significantly increased the growth of this alga and its cellular macro-molecules. While, treatment above this concentration with NaCl significantly inhibited the growth and cellular macro-molecules. On the other hand, treatment by NaCl at the pre-lethal concentration (300 mg L−1) had different effects on its detected amino acids. Whereas, Asp. Acid, Pro, Cys, Val, Iso-leu, leu, Phe.ala and Lys were slightly stimulated with salinization treatment. On contrast the levels of amino acids: Thr, Ser, Glu.acid, Gly, Ala, Mth, His and Arg were markedly inhibited. Ultrastructure examination of treated S. bijugatus by 300 mg L−1 of NaCl for 8 days showed increase of starch granules, shrinkage of cell contents and thickening of cell wall. The recorded data indicated also that treatment by wastewater with all concentrations led to stimulatory effects on their growth and cellular macro-molecules except at 100% wastewater which had inhibitory effects on Asp., Gly., Thr., Ser., Pro., Glu., Ala., Meth., and Cyst., of S. bijugatus. Also, wastewater induced a slight change in the treated S. bijugatus as elevation in starch granules and presence of thylakoid membranes although not clear as in the control.



中文翻译:

盐化和废水对双栅藻生长和部分细胞含量的影响。

本研究的目的是利用 TEM 确定盐化和废水胁迫对单细胞绿藻Scendesmus bijugatus的生长、某些细胞含量(总可溶性蛋白质、总可溶性碳水化合物、核酸和氨基酸组成)和超微结构的影响。用 10 和 50 mg L -1的 NaCl处理S. bijugatus显着增加了这种藻类及其细胞大分子的生长。而高于该浓度的 NaCl 处理会显着抑制生长和细胞大分子。另一方面,致死前浓度(300 mg L -1)的 NaCl 处理对其检测到的氨基酸有不同的影响。而,阿斯普。Acid、Pro、Cys、Val、Iso-leu、leu、Phe.ala 和 Lys 受到盐化处理的轻微刺激。相反,氨基酸的水平:Thr、Ser、Glu.acid、Gly、Ala、Mth、His 和 Arg 被显着抑制。经300 mg L -1 NaCl处理8天的S. bijugatus超微结构检查显示,淀粉颗粒增加,细胞内容物收缩,细胞壁增厚。记录的数据还表明,除100%废水对Asp.、Gly.、Thr.、Ser.、Pro.、Glu有抑制作用外,所有浓度的废水处理都会对其生长和细胞大分子产生刺激作用。 .、Ala.、Meth. 和 Cyst.,S. bijugatus。此外,废水引起了处理过的S. bijugatus的轻微变化,即淀粉颗粒的升高和类囊体膜的存在,尽管与对照相比并不明显。

更新日期:2020-05-20
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