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Manipulation of living cells with 450 nm laser photobiomodulation.
Journal of Photochemistry and Photobiology B: Biology ( IF 5.4 ) Pub Date : 2020-05-20 , DOI: 10.1016/j.jphotobiol.2020.111896
Wenzhi Hu 1 , Yuehou Zhang 2 , Bingmin Li 1 , Qiankun Li 3 , Kui Ma 4 , Cuiping Zhang 4 , Xiaobing Fu 5
Affiliation  

Increasing studies demonstrated that photobiomodulation (PBM) influenced specific biological effects in cells, tissues and organs, and these effects rely on the production of light irradiation. In this study, we aimed to precisely manipulate the spatial arrangement of adhesion cells in a traditional culture condition with 450 nm low intensity laser. Through 450 nm laser PBM, the adhesion of the cultured cells was significantly improved and resisted the digestion of 0.1% trypsin. Combined with a computer aided design system (CAD) and computer numerical control (CNC) system, the designed laser irradiation pattern induced the specific cell micropattern in the culture dish. RNA sequencing and biochemical experiments confirmed that the 450 nm laser prompted low-density lipoprotein (LDL) bonding to the cell surface and induced lipid peroxidation, which crosslinked and modified the protein molecules on the irradiated cell surface. In this way, the peroxidation product-modified proteins resisted trypsin proteolysis, ultimately leading to a differential detachment between the irradiated and non-irradiated cells under trypsin treatment. This convenient method did not require special biomaterial processing, has no impact on cell viability and functions, and required no changes to the conventional cell culture conditions. The photo-induced cell capturing is a great complement to existing tools by providing spatial resolution.



中文翻译:

用450 nm激光光生物调节技术处理活细胞。

越来越多的研究表明,光生物调节(PBM)影响细胞,组织和器官中的特定生物学效应,这些效应依赖于光辐射的产生。在这项研究中,我们旨在使用450 nm低强度激光在传统培养条件下精确控制粘附细胞的空间排列。通过450 nm激光PBM,可显着改善培养细胞的粘附力,并抵抗0.1%胰蛋白酶的消化。结合计算机辅助设计系统(CAD)和计算机数控(CNC)系统,设计的激光照射模式可在培养皿中诱导特定的细胞微模式。RNA测序和生化实验证实450 nm激光会促使低密度脂蛋白(LDL)结合到细胞表面并诱导脂质过氧化,交联并修饰了被照射细胞表面上的蛋白质分子。以这种方式,过氧化产物修饰的蛋白质抵抗胰蛋白酶水解,最终导致在胰蛋白酶处理下被照射细胞和未照射细胞之间的差异脱离。这种方便的方法不需要特殊的生物材料处理,对细胞活力和功能没有影响,并且不需要改变常规的细胞培养条件。通过提供空间分辨率,光诱导的细胞捕获是对现有工具的重要补充。最终导致在胰蛋白酶处理下,受辐照的细胞与未辐照的细胞之间出现差异性分离。这种方便的方法不需要特殊的生物材料处理,对细胞活力和功能没有影响,并且不需要改变常规的细胞培养条件。通过提供空间分辨率,光诱导的细胞捕获是对现有工具的重要补充。最终导致在胰蛋白酶处理下,受辐照的细胞与未辐照的细胞之间出现差异性分离。这种方便的方法不需要特殊的生物材料处理,对细胞活力和功能没有影响,并且不需要改变常规的细胞培养条件。通过提供空间分辨率,光诱导的细胞捕获是对现有工具的重要补充。

更新日期:2020-05-20
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