Geniposide (GP), extracted from a traditional Chinese herb Gardenia jasminoides, has extensive pharmacological effects. But the effects and the potential mechanisms of GP on myocardial ischemia/reperfusion (I/R) injury are poorly understood. In present study, we investigated the effect of GP on myocardial I/R injury in vivo and hypoxia/reoxygenation (H/R) in vitro respectively, and its mechanism. The results showed that GP reduced myocardial infarct size, alleviated acute myocardial injury, improved cardiac function, regulated apoptosis-related proteins and inhibited apoptosis. In vitro experiments revealed that GP enhanced the cell viability, regulated apoptosis-related proteins and prevented cell apoptosis during H/R in H9c2 cells. GP inhibited the expression of autophagy-related proteins and autophagosome accumulation both in vivo and in vitro. The effects of GP were blocked by rapamycin (RAPA) administration. In summary, our results showed that GP protected against myocardial I/R injury and involved inhibition of autophagy, which might be through activating AKT/mTOR signaling pathways.

ip子苷（GP）提取自传统的Garden子茉莉，具有广泛的药理作用。但是，GP对心肌缺血/再灌注（I / R）损伤的影响及其潜在机制尚不清楚。在本研究中，我们调查对心肌I / R损伤GP的效果的体内和缺氧/复氧（H / R）在体外分别，其作用机制。结果表明，GP减少了心肌梗死面积，减轻了急性心肌损伤，改善了心功能，调节了凋亡相关蛋白并抑制了凋亡。体外实验表明，GP可增强H9c2细胞在H / R过程中的细胞活力，调节凋亡相关蛋白并阻止细胞凋亡。GP在体内和体外均抑制自噬相关蛋白的表达和自噬体的积累。雷帕霉素（RAPA）的给药阻断了GP的作用。总之，我们的结果表明GP可以保护心肌免受I / R损伤，并抑制自噬，这可能是通过激活AKT / mTOR信号通路来实现的。