The ubiquitin specific peptidase (USP) family is involved in many life processes, of which antiviral is also an important basic function. One of the more important ways is to activate interferon. In this study, we reported the antiviral function of the ubiquitin specific peptidase 5(USP5) gene in zebrafish. Evolutionary and comparative protein sequence analysis of the USP5 was performed. The localization of USP5 in FHM cells cytoplasm was determined. Overexpression of USP5 significantly evoked higher expression of mRNA that encode IFNφ1 and ISGs, the promoteractivities of IFNφ1 and IFNstimulated response element (ISRE) were augmented likewise. USP5 was also able to enhance the expression of RIG-I and activate higher levels of IFNφ1 stimulated by Poly (I: C). Viral infection and interference tests demonstrated that USP5 inhibited the replication of SVCV in vitro. In summary, this study reveals that USP5 is able to activate higher levels of interferon by increasing RIG-I protein levels, and thus implement antivirus functions.

中文翻译：

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斑马鱼泛素特异性肽酶5（USP5）通过增加RIG-1的表达来激活干扰素对该病毒的抗性。

泛素特异性肽酶（USP）家族参与许多生命过程，其中抗病毒也是重要的基本功能。更重要的方法之一是激活干扰素。在这项研究中，我们报道了在斑马鱼中泛素特异性肽酶5（USP5）基因的抗病毒功能。进行了USP5的进化和比较蛋白序列分析。确定USP5在FHM细胞胞质中的定位。USP5的过表达显着引起编码IFNφ1和ISG的mRNA的更高表达，同样提高了IFNφ1和IFN刺激的响应元件（ISRE）的启动子活性。USP5还能够增强RIG-1的表达并激活Poly（I：C）刺激的更高水平的IFNφ1。病毒感染和干扰试验表明，USP5在体外抑制了SVCV的复制。总之，这项研究表明，USP5能够通过增加RIG-I蛋白水平来激活更高水平的干扰素，从而实现抗病毒功能。