Narcissus tazetta L., a bulbous plant belongs to the Amaryllidaceae family, contains alkaloid galantamine (GAL) with acetylcholinesterase inhibitory activity which has been recently considered to treat Alzheimer’s disease (AD). In the current work, the effect of photoperiod (16/8 h light/dark and 24 h dark) and various concentrations of NAA, BAP, and GA₃ (0, 0.5, 1 and 2 mg l^‒1) on the in vitro mass bulblet regeneration of N. tazetta was studied. The GAL production ability of the regenerated bulblets was assessed by HPLC-UV-MS. Light treatments significantly affected the number of bulblet and leaf, the ratio of bulblet/leaf, and leaf length. The maximum number of bulblet (31.0 ± 1.58) and leaf (13.3 ± 1.33) was recorded from the cultures fortified with NAA and BAP (2 mg l^‒1) kept in 16/8 h light/dark, while the maximum leaf length (2.1 ± 0.92 cm) was measured on the MS medium containing 0.5 mg l^‒1 NAA and 2 mg l^‒1 BAP incubated in the same photoperiod. The average ratio of bulblet proliferation per explant was significantly different between studied photoperiod (1.1 ± 0.86) and 24 h dark (0.62 ± 0.31). The regenerated bulblets contained 40 and 20 µg g^‒1 DW GAL underexposed photoperiod and 24 h dark, respectively. This information could be useful in the commercial production of GAL as a valuable anti-AD compound through in vitro mass bulblet proliferation of N. tazetta.

Narcissus tazetta L. 是石蒜科的球茎植物，含有具有乙酰胆碱酯酶抑制活性的生物碱雪花胺 (GAL)，最近被认为可治疗阿尔茨海默病 (AD)。在目前的工作中，光周期（16/8 h 光照/黑暗和 24 h 黑暗）和不同浓度的 NAA、BAP 和 GA ₃（0、0.5、1 和 2 mg l ^-1）对体外的影响N. tazetta的大规模鳞茎再生进行了研究。通过 HPLC-UV-MS 评估再生鳞茎的 GAL 生产能力。光处理显着影响鳞茎和叶片的数量、鳞茎/叶片的比例和叶片长度。在 16/8 小时光照/黑暗条件下，用 NAA 和 BAP (2 mg l ^-1 )强化的培养物记录了小鳞茎 (31.0 ± 1.58) 和叶片 (13.3 ± 1.33) 的最大数量，而最大叶片长度 ( 2.1 ± 0.92 cm) 在含有 0.5 mg l ^-1 NAA 和 2 mg l ^-1 BAP 的 MS 培养基上在相同的光周期中孵育。在研究的光周期 (1.1 ± 0.86) 和 24 小时黑暗 (0.62 ± 0.31) 之间，每个外植体的鳞茎增殖的平均比率显着不同。再生鳞茎含有 40 和 20 µg g ^‒1DW GAL 曝光不足的光周期和 24 小时黑暗，分别。该信息可用于通过N. tazetta的体外大规模鳞茎增殖将 GAL 作为有价值的抗 AD 化合物进行商业生产。