Ajuga xylorrhiza Kit Tan is a rare endemic perennial species growing in the stony habitats in Southeastern Anatolia region, Turkey. An efficient and reproducible protocol has been developed for micropropagation of endangered Ajuga xylorrhiza. Shoots were cultured on Murashige and Skoog (MS) basal medium, supplemented with various concentrations of 6-benzyl amino purine (BAP), thidiazuron (TDZ), and kinetin (Kin). The optimal shoot formation was with the medium containing 0.125 mg L⁻¹ BAP (7.91 shoots per explant), and 0.25 mg L⁻¹ Kin (4.9 shoots per explant) was recorded. The best result in terms of shoot length (3.43 to 3.63 cm) was obtained from the Kin treatments. Kin parameters were more effective on shoot length and multiple shoot production than BAP and TDZ. For rooting, regenerated shoots were transferred to 1/4 strength MS medium containing two different concentrations of indole acetic acid (IAA) and α-naphthalenetic acid (NAA) at 0 (control), 0.5, or 1.0 mg L⁻¹.The best results in terms of the number of roots per shoot were obtained from the medium supplemented with 0.5 mg L⁻¹ IAA (3.10 roots per explant). Rooted plantlets were transferred to soil for acclimization with 100% success rate.

Ajuga xylorrhiza Kit Tan是一种稀有的多年生物种，生长在土耳其东南安纳托利亚地区的石质生境中。已经开发了一种有效的和可复制的方案，用于濒危的木枣的微繁殖。将芽在Murashige和Skoog（MS）基础培养基上培养，并补充各种浓度的6-苄基氨基嘌呤（BAP），噻唑酮（TDZ）和激动素（Kin）。最佳芽形成是在含有0.125 mg L ^-1 BAP（每个外植体为7.91个芽）和0.25 mg L ^-1的培养基中进行的。记录Kin（每个外植体4.9枝）。从Kin处理中获得了最好的枝条长度（3.43至3.63 cm）。Kin参数比BAP和TDZ对枝条长度和多枝产生更有效。为了生根，将再生的芽转移到1/4强度MS培养基中，该培养基含有0（对照），0.5或1.0 mg L ^-1的两种不同浓度的吲哚乙酸（IAA）和α-萘甲酸（NAA）。从补充0.5 mg L ^-1 IAA（每个外植体3.10根）的培养基中获得了每根的根数结果。将生根的小植株转移到土壤中进行驯化，成功率达100％。