In the last decade, zebrafish has been used as a model for the study of several human skin diseases. The epidermis of Danio rerio is composed of keratinocytes and two types of secretory cells: mucous cells and club cells. Club cells have multiple biological functions and among them may be important in the protection against ultraviolet damage through the proliferative response or through the increased production of protective substances. Calcium-binding proteins such as calbindin D28K and calretinin are used as markers of nervous and enteric nervous systems, but they are present in numerous other cells. These proteins are involved in a wide variety of cell activities, such as cytoskeletal organization, cell motility and differentiation, cell cycle regulation and neuroprotective function. In this study we demonstrated, for the first time, the presence of calretinin and calbindin D28K in skin club cells of Danio rerio exposed to different wavelengths by immunohistochemistry analysis. Exposure to white-blue light and blue light causes the expression and colocalization of calbindin-D28K and calretinin. These proteins were moderately expressed and no colocalization was observed in the club cells of the control fish. In zebrafish exposed to continuous darkness for 10 days, in the club cells the two antibodies did not detect any proteins specifically. These results demonstrate that calbindin and calretinin could be involved in the pathophysiology of skin injury due to exposure to short-wavelength visible light spectrums.

中文翻译：

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光照对成年斑马鱼皮肤俱乐部细胞中Calretinin和Calbindin表达的影响。

在过去的十年中，斑马鱼已被用作研究几种人类皮肤疾病的模型。达尼奥·里奥的表皮由角质形成细胞和两种分泌细胞组成：粘液细胞和俱乐部细胞。俱乐部细胞具有多种生物学功能，其中可能在通过增殖反应或增加保护性物质的产生来防止紫外线损伤中发挥重要作用。钙结合蛋白（如钙结合蛋白D28K和钙网蛋白）被用作神经和肠神经系统的标志物，但它们存在于许多其他细胞中。这些蛋白质参与多种细胞活动，例如细胞骨架组织，细胞运动和分化，细胞周期调节和神经保护功能。在这项研究中，我们首次展示了 通过免疫组织化学分析，在暴露于不同波长的雷氏达尼奥皮肤俱乐部细胞中钙网蛋白和钙结合蛋白D28K的存在。暴露于白蓝光和蓝光下会导致钙结合蛋白-D28K和钙网蛋白的表达和共定位。这些蛋白适度表达，在对照鱼的俱乐部细胞中未观察到共定位。在暴露于连续黑暗中10天的斑马鱼中，在俱乐部细胞中，这两种抗体没有特异性检测到任何蛋白质。这些结果表明，由于暴露于短波可见光谱，钙调蛋白和钙调蛋白可能与皮肤损伤的病理生理有关。暴露在白蓝光和蓝光下会导致钙结合蛋白-D28K和钙网蛋白的表达和共定位。这些蛋白适度表达，在对照鱼的俱乐部细胞中未观察到共定位。在连续黑暗中暴露10天的斑马鱼中，在俱乐部细胞中，这两种抗体没有特异性检测到任何蛋白质。这些结果表明，由于暴露于短波可见光谱，钙调蛋白和钙调蛋白可能与皮肤损伤的病理生理有关。暴露于白蓝光和蓝光下会导致钙结合蛋白-D28K和钙网蛋白的表达和共定位。这些蛋白适度表达，在对照鱼的俱乐部细胞中未观察到共定位。在暴露于连续黑暗中10天的斑马鱼中，在俱乐部细胞中，这两种抗体没有特异性检测到任何蛋白质。这些结果表明，由于暴露于短波可见光谱，钙调蛋白和钙调蛋白可能与皮肤损伤的病理生理有关。在俱乐部细胞中，这两种抗体没有特异性检测任何蛋白质。这些结果表明，由于暴露于短波可见光谱，钙调蛋白和钙调蛋白可能与皮肤损伤的病理生理有关。在俱乐部细胞中，两种抗体没有特异性检测任何蛋白质。这些结果表明，由于暴露于短波可见光谱，钙调蛋白和钙调蛋白可能与皮肤损伤的病理生理有关。