当前位置: X-MOL 学术Cell. Mol. Neurobiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Differential Regulation of Wnt Signaling Components During Hippocampal Reorganization After Entorhinal Cortex Lesion.
Cellular and Molecular Neurobiology ( IF 4 ) Pub Date : 2020-05-20 , DOI: 10.1007/s10571-020-00870-x
Lizbeth García-Velázquez 1 , Clorinda Arias 1
Affiliation  

Entorhinal cortex lesions have been established as a model for hippocampal deafferentation and have provided valuable information about the mechanisms of synapse reorganization and plasticity. Although several molecules have been proposed to contribute to these processes, the role of Wnt signaling components has not been explored, despite the critical roles that Wnt molecules play in the formation and maintenance of neuronal and synaptic structure and function in the adult brain. In this work, we assessed the reorganization process of the dentate gyrus (DG) at 1, 3, 7, and 30 days after an excitotoxic lesion in layer II of the entorhinal cortex. We found that cholinergic fibers sprouted into the outer molecular layer of the DG and revealed an increase of the developmental regulated MAP2C isoform 7 days after lesion. These structural changes were accompanied by the differential regulation of the Wnt signaling components Wnt7a, Wnt5a, Dkk1, and Sfrp1 over time. The progressive increase in the downstream Wnt-regulated elements, active-β-catenin, and cyclin D1 suggested the activation of the canonical Wnt pathway beginning on day 7 after lesion, which correlates with the structural adaptations observed in the DG. These findings suggest the important role of Wnt signaling in the reorganization processes after brain lesion and indicate the modulation of this pathway as an interesting target for neuronal tissue regeneration.

中文翻译:

内嗅皮层损伤后海马重组过程中 Wnt 信号成分的差异调节。

内嗅皮层病变已被建立为海马传入传入的模型,并提供了有关突触重组和可塑性机制的宝贵信息。尽管已经提出了几种分子参与这些过程,但 Wnt 信号组件的作用尚未被探索,尽管 Wnt 分子在成人大脑中神经元和突触结构和功能的形成和维持中发挥着关键作用。在这项工作中,我们评估了在内嗅皮层 II 层发生兴奋性毒性病变后 1、3、7 和 30 天时齿状回 (DG) 的重组过程。我们发现胆碱能纤维萌发到 DG 的外分子层,并在病变 7 天后显示发育调节的 MAP2C 异构体​​增加。这些结构变化伴随着 Wnt 信号成分 Wnt7a、Wnt5a、Dkk1 和 Sfrp1 随时间的差异调节。下游 Wnt 调节元件、活性-β-连环蛋白和细胞周期蛋白 D1 的逐渐增加表明典型 Wnt 通路在损伤后第 7 天开始激活,这与在 DG 中观察到的结构适应相关。这些发现表明 Wnt 信号在脑损伤后重组过程中的重要作用,并表明该通路的调节是神经元组织再生的一个有趣目标。和细胞周期蛋白 D1 表明在损伤后第 7 天开始激活经典 Wnt 通路,这与在 DG 中观察到的结构适应相关。这些发现表明 Wnt 信号在脑损伤后重组过程中的重要作用,并表明该通路的调节是神经元组织再生的一个有趣目标。和细胞周期蛋白 D1 表明在损伤后第 7 天开始激活经典 Wnt 通路,这与在 DG 中观察到的结构适应相关。这些发现表明 Wnt 信号在脑损伤后重组过程中的重要作用,并表明该通路的调节是神经元组织再生的一个有趣目标。
更新日期:2020-05-20
down
wechat
bug