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Irvingia excelsa Mildbr. ethanolic extract displays in vitro cytotoxic effects on prostate cancer cells and prevents benign prostatic hyperplasia in Wistar rats
Biologia ( IF 1.5 ) Pub Date : 2020-05-20 , DOI: 10.2478/s11756-020-00511-0
Dieudonné Njamen , Berlise Y. Bakam , Jochen Rutz , Sebastian Maxeiner , Charline F. Awounfack , Derek T. Ndinteh , Eva Jüngel , Stéphane Zingue , Roman A. Blaheta

Despite much recent progress in prostate research, Benign prostatic hyperplasia (BPH) and prostate cancer continue to represent major causes of morbidity and mortality in men. In our ongoing search of natural alternatives to treat these prostate diseases, ethnobotanical reports indicated the use of Irvingia excelsa in the treatment of prostate inflammation and tumors in Cameroun. To investigate this claim, the cytotoxicity of the plant extract was investigated using MTT assay in three prostate cancer cell lines (LNCaP, DU145 and PC3). In addition, tumor cell growth/proliferation, clonogenic assay and cell cycle analyses were also assessed. In vivo, testosterone-induced benign prostatic hyperplasia (BPH) rats were treated orally with the extract (75, 150 and 300 mg/kg BW) or permixon (50 mg/kg) as standard for 28 days. Irvingia excelsa extract at concentrations of 50, 100 and 500 μg/mL significantly inhibited LNCaP, DU145 and PC3 prostate cancer cell growth and proliferation. The number of PC3 and DU145 clones was considerably diminished in a concentration-dependent manner. In addition, there was increase in the number of S-phase cells and decrease in the number of G0/G1 cells. Meanwhile results from in vivo study showed that I. excelsa extract significantly reduced prostate relative wet weight/volume and prostatic epithelium thickness at all tested doses with an optimal effect at 300 mg/kg BW. These results suggest that I. excelsa extract has in vitro and in vivo antiproliferative properties, which could justify its use by Cameroonian people to alleviate or treat prostate inflammation and tumors.



中文翻译:

Irvingia excelsa Mildbr。乙醇提取物对Wistar大鼠的前列腺细胞具有体外细胞毒性作用并防止前列腺增生

尽管最近在前列腺研究方面取得了很大进展,但良性前列腺增生(BPH)和前列腺癌仍然是男性发病率和死亡率的主要原因。在我们不断寻找可替代这些前列腺疾病的天然替代品的过程中,民族植物学报告指出,使用欧文雅Ervingia excelsa)在喀麦隆治疗前列腺炎症和肿瘤。为了研究这一要求,使用MTT测定法在三种前列腺癌细胞系(LNCaP,DU145和PC3)中研究了植物提取物的细胞毒性。此外,还评估了肿瘤细胞的生长/增殖,克隆形成测定和细胞周期分析。在体内,以提取物(75、150和300 mg / kg BW)或permixon(50 mg / kg)作为标准品口服治疗睾丸激素诱导的良性前列腺增生(BPH)大鼠28天。欧文雅浓度为50、100和500μg/ mL的提取物显着抑制LNCaP,DU145和PC3前列腺癌细胞的生长和增殖。PC3和DU145克隆的数量以浓度依赖的方式大大减少。另外,S期细胞数目增加而G0 / G1细胞数目减少。同时,来自体内研究的结果表明,excelsa提取物在所有测试剂量下均显着降低了前列腺相对湿重/体积和前列腺上皮厚度,在300 mg / kg体重下具有最佳效果。这些结果表明,excelsa提取物具有体外和体内的抗增殖特性,可以证明喀麦隆人使用它来减轻或治疗前列腺炎和肿瘤。

更新日期:2020-05-20
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