Salinity was shown to impact the formation of 2-acetyl-1-pyrroline (2-AP), the key flavour compound in aromatic rice cultivars. To study the effects of saline application to seeds on 2-AP biosynthesis in aromatic rice and to further investigate the enzymatic and molecular mechanisms involved, the present study was conducted with nine treatments. The seeds of three aromatic rice cultivars were primed or soaked in 0.01, 0.02, 0.05 and 0.10 mM sodium chloride solutions, and the seeds that were both primed and soaked in distilled water were considered the control group. Then, the seedlings were allowed to grow in an artificial climate box. The results showed that both priming and soaking seeds with saline significantly increased the 2-AP content in aromatic rice seedlings. The highest 2-AP content was recorded in the seeds primed with 0.10 mM sodium chloride. In the seeds treated with saline, increased amounts of proline, P5C and Δ1-pyrroline were observed and resulted in enhanced 2-AP biosynthesis through induced gene expression (P5CS1, P5CS2 and PRODH) and activities of enzymes including PDH and P5CS. In summary, application of saline to seeds increased 2-AP content in aromatic rice seedlings by up-regulating the related precursors contents, enzymes activities and genes expression.

结果表明，盐度会影响2-乙酰基-1-吡咯啉（2-AP）的形成，2-乙酰基-1-吡咯啉是香米中的关键风味化合物。为了研究盐溶液对香米中2-AP生物合成的影响，并进一步研究涉及的酶促和分子机制，本研究共进行了九种处理。将三种芳香稻品种的种子在0.01、0.02、0.05和0.10 mM氯化钠溶液中涂底料或浸泡，将涂底料和浸泡在蒸馏水中的种子视为对照组。然后，使幼苗在人工气候箱中生长。结果表明，用生理盐水引发和浸种均可显着增加芳香稻幼苗中2-AP的含量。记录为0的种子中2-AP含量最高。10 mM氯化钠。在用盐水处理的种子中，观察到脯氨酸，P5C和Δ1-吡咯啉的含量增加，并通过诱导的基因表达（P5CS1，P5CS2和PRODH）以及包括PDH和P5CS在内的酶的活性增强了2-AP的生物合成。总之，通过上调相关的前体含量，酶活性和基因表达，在种子上施用生理盐水可以增加香米中2-AP的含量。