Ultrafast reversible DNA/RNA photo-cross-linking is a powerful tool for regulating the target strand in living cells. In particular, 3-cyanovinylcarbazole (^CNVK) and 3-cyanovinylcarbazole modified by D-threoninol (^CNVD) can photo-cross-link to pyrimidine bases within a few seconds of photoirradiation. However, these photo-cross-linkers can only cross-link to the counter base if it is adjacent to the 5′-side (−1 position). In this study, we synthesized novel photo-cross-linkers with varying linker lengths capable of photo-cross-linking with pyrimidine bases at locations other than the −1 position via click chemistry. The photo-cross-linking site was dependent on linker length.

中文翻译：

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接头长度对通过点击化学经由[2 + 2]光环加成反应介导的光交联位置的影响。

超快速可逆DNA / RNA光交联是调节活细胞中靶链的强大工具。特别地，通过D-苏氨酸（^CNV D）修饰的3-氰基乙烯基咔唑（^CNV K）和3-氰基乙烯基咔唑可以在光辐照的几秒钟内光交联到嘧啶碱基上。但是，这些光交联剂仅在与5'侧（-1位置）相邻时才可以与对接基交联。在这项研究中，我们合成了具有不同接头长度的新型光交联剂，这些光交联剂能够通过点击化学与除-1位置以外的嘧啶碱基进行光交联。光交联位点取决于接头长度。