BACKGROUND We evaluated the functional capacity of plantaricin-producing Lactobacillus plantarum SF9C and S-layer-carrying Lactobacillus brevis SF9B to withstand gastrointestinal transit and to compete among the gut microbiota in vivo. Considering the probiotic potential of Lb. brevis SF9B, this study aims to investigate the antibacterial activity of Lb. plantarum SF9C and their potential for in vivo colonisation in rats, which could be the basis for the investigation of their synergistic functionality. RESULTS A plantaricin-encoding cluster was identified in Lb. plantarum SF9C, a strain which efficiently inhibited the growth of Listeria monocytogenes ATCC® 19111™ and Staphylococcus aureus 3048. Homology-based three-dimensional (3D) structures of SF9C plantaricins PlnJK and PlnEF were predicted using SWISS-MODEL workspace and the helical wheel representations of the plantaricin peptide helices were generated by HELIQUEST. Contrary to the plantaricin-producing SF9C strain, the S-layer-carrying SF9B strain excluded Escherichia coli 3014 and Salmonella enterica serovar Typhimurium FP1 from the adhesion to Caco-2 cells. Finally, PCR-DGGE analysis of the V2-V3 regions of the 16S rRNA gene confirmed the transit of the two selected lactobacilli through the gastrointestinal tract (GIT). Microbiome profiling via the Illumina MiSeq platform revealed the prevalence of Lactobacillus spp. in the gut microbiota of the Lactobacillus-treated rats, even on the 10th day after the Lactobacillus application, compared to the microbiota of the healthy and AlCl3-exposed rats before Lactobacillus treatment. CONCLUSION The combined application of Lb. plantarum SF9C and Lb. brevis SF9B was able to influence the intestinal microbiota composition in rats, which was reflected in the increased abundance of Lactobacillus genus, but also in the altered abundances of other bacterial genera, either in the model of healthy or aberrant gut microbiota of rats. The antibacterial activity and capacity to withstand in GIT conditions contributed to the functional aspects of SF9C and SF9B strains that could be incorporated in the probiotic-containing functional foods with a possibility to positively modulate the gut microbiota composition.

中文翻译：

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生产plant那霉素的植物乳杆菌SF9C和携带S层的短乳杆菌SF9B抵抗胃肠道运输的功能能力。

背景技术我们评估了生产plant那霉素的植物乳杆菌SF9C和携带S层的短乳杆菌SF9B抵抗胃肠道转运并在体内肠道菌群之间竞争的功能能力。考虑到Lb的益生菌潜力。brevis SF9B，本研究旨在研究Lb的抗菌活性。植物SF9C及其在大鼠体内定植的潜力，可能是研究其协同功能的基础。结果在Lb中鉴定出了一种植物素编码簇。plantarum SF9C，一种有效抑制单核细胞增生李斯特菌ATCC®19111™和金黄色葡萄球菌3048的菌株。使用SWISS-MODEL工作区预测了SF9C植物霉素PlnJK和PlnEF的基于同源性的三维（3D）结构，并通过HELIQUEST生成了植物霉素肽螺旋的螺旋轮表示。与生产plant那霉素的SF9C菌株相反，携带S层的SF9B菌株从对Caco-2细胞的粘附中排除了大肠杆菌3014和肠炎沙门氏菌血清鼠伤寒沙门氏菌FP1。最后，对16S rRNA基因的V2-V3区域进行PCR-DGGE分析，确认了两个选定的乳酸菌通过胃肠道（GIT）的转运。通过Illumina MiSeq平台进行的微生物组分析显示了乳酸杆菌属的流行。在乳杆菌治疗的大鼠的肠道菌群中，甚至在应用乳杆菌后的第10天，与乳酸菌治疗前健康和暴露于AlCl3的大鼠的菌群相比。结论Lb的联合应用。车前草SF9C和Lb。brevis SF9B能够影响大鼠的肠道菌群组成，这反映在乳酸杆菌属的丰度增加，但在健康或异常肠道菌群模型中其他细菌属的丰度也发生了变化。抗菌活性和在GIT条件下的耐受能力有助于SF9C和SF9B菌株的功能，这些菌株可以掺入含益生菌的功能性食品中，并可能积极调节肠道菌群组成。brevis SF9B能够影响大鼠的肠道菌群组成，这反映在乳酸杆菌属的丰度增加，但在健康或异常肠道菌群模型中其他细菌属的丰度也发生了变化。抗菌活性和在GIT条件下的耐受能力有助于SF9C和SF9B菌株的功能，这些菌株可以掺入含益生菌的功能性食品中，并可能积极调节肠道菌群组成。brevis SF9B能够影响大鼠的肠道菌群组成，这反映在乳酸杆菌属的丰度增加，但在健康或异常肠道菌群模型中其他细菌属的丰度也发生了变化。抗菌活性和在GIT条件下的耐受能力有助于SF9C和SF9B菌株的功能，这些菌株可以掺入含益生菌的功能性食品中，并可能积极调节肠道菌群组成。