Bioinspired by the morphology of osteoclast-resorbed bone surfaces, we prepared a calcium-doped titanium phosphate (Ca-TiP) coating, which consists of a nanofibrous network, on titanium (Ti) substrate via a simple two-step hydrothermal method, trying to mimic natural bone compositionally and microstructurally. The in vitro studies show that the Ca-TiP coating with synergistic features of nanofibrous biomimetic topography and surface chemistry could elicit intensively osteogenic behavior and responses including enhanced cell adhesion, spreading, and proliferation as well as alkaline phosphatase (ALP) activity and up-regulated expression of bone-related genes, which inevitably benefit the formation of new bone and the quality of osseointegration. When the two control groups are compared in vivo, the significantly improved new bone formation in the early stage and the much stronger interfacial bonding with the surrounding bone for Ca-TiP coating suggest that Ca-TiP coating modified Ti implants hold great potential for orthopedic and dental applications.

中文翻译：

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具有纳米纤维微结构的钙掺杂TiP涂层的生物启发加工，以加速骨整合。

受破骨细胞吸收的骨表面形态学的启发，我们通过简单的两步水热法在钛（Ti）基底上制备了由纳米纤维网络组成的钙掺杂的磷酸钛（Ca-TiP）涂层，在结构和微观结构上模仿天然骨骼。的体外研究表明，与纳米纤维仿生形貌和表面化学的协同功能的Ca-TIP涂层能引起强烈的成骨行为和响应，包括增强的细胞粘附，扩频和增殖以及碱性磷酸酶（ALP）活性和上调的骨相关基因的表达，这不可避免地有利于新骨的形成和骨整合的质量。在体内比较两个对照组时，早期显着改善了新骨的形成，Ca-TiP涂层与周围骨骼的界面结合更牢固，这表明Ca-TiP涂层改性的Ti植入物在整形外科和牙科应用中具有巨大的潜力。