ABSTRACT Phelipanche aegyptiaca is an obligate holo-parasitic weedlacking a functional photosynthetic system, which subsists on roots of a wide range of host crops, causing severe losses in yield quality and quantity. The parasite and its host are connected through their vascular system, forming a unique ecological system that enables the exchange of various substances. In a previous study, it was suggested that endophytic bacteria, which naturally inhabit the internal tissues of plants, can also be transmitted from the parasitic weed to its host and vice versa. In the current study, we investigate the characteristics of a previously isolated Pseudomonas sp. PhelS10 strain using both biochemical and molecular methods. This isolate was obtained from tomato plant tissue and was able to reduce P. aegyptiaca parasitism, and thus it may serve as a biocontrol agent. Our results revealed that production of Pseudomonas aeruginosa quinolone signal (PQS) was 2.1 times higher than that of the standard Pseudomonas aeruginosa strain (PAO1), which contributed to a 22% higher biofilm formation capability. PhelS10 strain was detected in the xylem of tomato plants using FISH analysis. In addition, PhelS10 strain was found in the parasitic weed’s inner tissues, confirming the hypothesis that endophytic bacteria traffic between the host plant and its parasitic weed.

中文翻译：

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源自番茄 (Solanum lycopersicum L.) 的内生细菌 (Pseudomonas aeruginosa) 的特征及其寄生于寄生杂草 Phelipanche aegyptiaca 的能力

摘要 Phelipanche aegyptiaca 是一种专性全寄生杂草，缺乏功能性光合作用系统，可在多种寄主作物的根部生存，导致产量质量和数量的严重损失。寄生虫与其宿主通过血管系统相连，形成独特的生态系统，可以进行各种物质的交换。在之前的一项研究中，有人提出，天然栖息在植物内部组织中的内生细菌也可以从寄生杂草传播到其宿主，反之亦然。在当前的研究中，我们调查了先前分离的假单胞菌的特征。使用生化和分子方法的 PhelS10 菌株。这种分离物是从番茄植物组织中获得的，能够减少埃及疟原虫的寄生，因此它可以作为生物防治剂。我们的结果显示铜绿假单胞菌喹诺酮信号（PQS）的产生是标准铜绿假单胞菌菌株（PAO1）的 2.1 倍，这有助于将生物膜形成能力提高 22%。使用 FISH 分析在番茄植株的木质部中检测到 PhelS10 菌株。此外，在寄生杂草的内部组织中发现了 PhelS10 菌株，证实了内生细菌在寄主植物与其寄生杂草之间传播的假设。使用 FISH 分析在番茄植株的木质部中检测到 PhelS10 菌株。此外，在寄生杂草的内部组织中发现了 PhelS10 菌株，证实了内生细菌在寄主植物与其寄生杂草之间传播的假设。使用 FISH 分析在番茄植株的木质部中检测到 PhelS10 菌株。此外，在寄生杂草的内部组织中发现了 PhelS10 菌株，证实了内生细菌在寄主植物与其寄生杂草之间传播的假设。