Kisspeptins, a family of neuropeptide encoded by the Kiss1 gene, have emerged as crucial regulator of fertility and reproduction by regulating the hypothalamic–pituitary–gonadal axis. The present study was aimed to identify and associate SNPs in the KISS1 gene with reproductive traits in cattle of Indian origin. DNA samples collected from 300 individual cows of three Indian dairy breeds (Gir, Kankrej and Frieswal) of cattle were used in the study. The SNPs of KISS1 gene were identified with PCR‐RFLP and sequence analysis using two sets of primer pairs. A total of 5 SNPs were identified in the targeted region of which, two were selected for screening the population and association studies. The analysis revealed that genotypes of rs442633552G>A and rs42022871C>T had a significant association with dry period. The SNP rs42022871C>T also established significant role in milk production traits, and selection of TT‐genotyped animals will improve the reproduction and production potential of the animals.

中文翻译：

---

牛KISS1单核苷酸多态性与印度牛生殖性状的关联。

Kisspeptins是由Kiss1基因编码的神经肽家族，已通过调节下丘脑-垂体-性腺轴而成为生育和繁殖的重要调节剂。本研究旨在确定KISS1基因中的SNP并将其与印度原产牛的繁殖性状联系起来。该研究使用了从三个印度奶牛场（Gir，Kankrej和Frieswal）的300头母牛身上收集的DNA样本。通过PCR-RFLP鉴定KISS1基因的SNP，并使用两组引物对进行序列分析。在目标区域中总共鉴定出5个SNP，选择了两个进行人群和关联研究的筛选。分析表明，rs442633552G> A和rs42022871C> T的基因型与干旱时期有显着相关性。SNP rs42022871C>