Human periodontal ligament stem cells (hPDLSCs) are a promising source in regenerative medicine. Due to the complexity and heterogeneity of hPDLSCs, it is critical to isolate homogeneous hPDLSCs with high regenerative potential. In this study, p75 neurotrophin receptor (p75NTR) was used to isolate p75NTR⁺ and p75NTR⁻ hPDLSCs by fluorescence‐activated cell sorting. Differences in osteogenic differentiation among p75NTR⁺, p75NTR⁻ and unsorted hPDLSCs were observed. Differential gene expression profiles between p75NTR⁺ and p75NTR⁻ hPDLSCs were analysed by RNA sequencing. α1 Integrin (ITGA1) small interfering RNA and ITGA1‐overexpressing adenovirus were used to transfect p75NTR⁺ and p75NTR⁻ hPDLSCs. The results showed that p75NTR⁺ hPDLSCs demonstrated superior osteogenic capacity than p75NTR⁻ and unsorted hPDLSCs. Differentially expressed genes between p75NTR⁺ and p75NTR⁻ hPDLSCs were highly involved in the extracellular matrix‐receptor interaction signalling pathway, and p75NTR⁺ hPDLSCs expressed higher ITGA1 levels than p75NTR⁻ hPDLSCs. ITGA1 silencing inhibited the osteogenic differentiation of p75NTR⁺ hPDLSCs, while ITGA1 overexpression enhanced the osteogenic differentiation of p75NTR⁻ hPDLSCs. These findings indicate that p75NTR optimizes the osteogenic potential of hPDLSCs by up‐regulating ITGA1 expression, suggesting that p75NTR can be used as a novel cell surface marker to identify and purify hPDLSCs to promote their applications in regenerative medicine.

中文翻译：

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p75NTR通过上调α1整合素表达来优化人牙周膜干细胞的成骨潜能。

人牙周膜干细胞（hPDLSCs）是再生医学中有希望的来源。由于hPDLSC的复杂性和异质性，分离具有高再生潜力的同质hPDLSC至关重要。在这项研究中，使用p75神经营养因子受体（p75NTR）通过荧光激活细胞分选分离p75NTR ⁺和p75NTR ^- hPDLSCs。在p75NTR的中成骨细胞分化的差异⁺，p75NTR的^-观察和无序hPDLSCs。通过RNA测序分析p75NTR ⁺和p75NTR ^- hPDLSCs之间的差异基因表达谱。使用α1整合素（ITGA1）小干扰RNA和过表达ITGA1的腺病毒转染p75NTR⁺和p75NTR ^- hPDLSCs。结果表明的p75NTR ⁺ hPDLSCs证实优越的成骨能力比的p75NTR ^-和未分选的hPDLSCs。p75NTR ⁺和p75NTR ^- hPDLSCs之间差异表达的基因高度参与细胞外基质-受体相互作用的信号通路，并且p75NTR ⁺ hPDLSCs的ITGA1水平高于p75NTR ^- hPDLSCs。ITGA1沉默抑制p75NTR ⁺ hPDLSCs的成骨分化，而ITGA1过表达增强p75NTR ^- hPDLSCs的成骨分化。 这些发现表明，p75NTR可通过上调ITGA1表达来优化hPDLSC的成骨潜能，这表明p75NTR可用作鉴定和纯化hPDLSC的新型细胞表面标志物，以促进其在再生医学中的应用。