Growth rate (GR), esterase activity (EA), membrane potential (MP), and DNA content were measured by flow cytometry to test if this powerful tool could be included in risk assessment and monitoring programs. This study tests a battery of endpoints that were measured on Scenedesmus (Acutodesmus) obliquus as model species, under high levels of injected CO₂ to be proposed as biomarkers of effect. New technologies such as carbon capture and storage (CCS) bring positive consequences on CO₂ mitigation strategies but also could have negative consequences if a CO₂ leakage occurs during injection. Under this scenario, pH might drop down to 4 pH units around the pipe where the leakage happens. The study focus on to ascertain if the endpoints fulfill the requirements such as sensitiveness, be timely and cost‐effective, be easy to measure and interpret, and be nondestructive, valuable attributes in effective biomarkers. CO₂ injected at high levels significantly affects the EA, the membrane polarization, as well as GRs. However the DNA content did not shown a clear response under this condition. In conclusion, the GR, the EA, and the MP analyzed by flow cytometry fulfilled the attributes mentioned above and are proposed as biomarkers of effect in CCS monitoring programs. © 2020 International Society for Advancement of Cytometry

中文翻译：

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CO2 注射下微藻生理变化的流式细胞术评估。

通过流式细胞术测量生长率 (GR)、酯酶活性 (EA)、膜电位 (MP) 和 DNA 含量，以测试这种强大的工具是否可以包含在风险评估和监测程序中。本研究中测试测定该端点上的电池栅藻（Acutodesmus）斜作为模型种类，下高水平注入的CO ₂将被提议作为的效果的生物标记。碳捕集与封存 (CCS) 等新技术对 CO ₂减排战略带来积极影响，但如果 CO ₂注射时发生泄漏。在这种情况下，发生泄漏的管道周围的 pH 值可能会下降到 4 个 pH 单位。该研究的重点是确定终点是否满足敏感度、及时且具有成本效益、易于测量和解释以及在有效生物标志物中具有非破坏性、有价值的属性等要求。以高水平注入的CO ₂显着影响 EA、膜极化以及 GR。然而，在这种情况下，DNA 含量没有显示出明确的反应。总之，通过流式细胞术分析的 GR、EA 和 MP 满足上述属性，并被提议作为 CCS 监测程序中效果的生物标志物。© 2020 国际细胞计量学促进会