Global infertility prevalence has been increasing in recent decades, mainly due to advanced reproductive age. Concerned women look for dietary supplements with antioxidant properties advertised as a natural way to increase fertility. Curcumin (CUR) is a polyphenol with antioxidant and anti-inflammatory properties. CUR elicits apoptotic cell death as evidenced in some tumor cells. In this work, the effect of CUR on granulosa cells (GC) was studied. GC surround the oocyte, providing nutrient exchange and hormone production, necessary for its development. COV434 cell line and primary human granulosa cells (hGC) cultures from patients undergoing Assisted Reproductive Technology (ART) were used. GC were treated with CUR (0.001-50 μM) at different times (24-72 h). Low concentrations of CUR showed an increase on cell viability. Likewise, it leads to a decrease in ROS/RNS formation after stress induction, suggesting a protective role. Changes in hormonal levels were not observed. In contrast, high concentrations of CUR triggered a reduction on cell viability and a programmed cell death mechanism. Allied to the above results, high doses of CUR affected hormonal function of GCs. Our work reinforces the benefits of dietary supplements, namely CUR, on the main functions of GC and, consequently, on reproductive success.

中文翻译：

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姜黄素对人类颗粒细胞的不同作用：除了其抗氧化作用。

近几十年来，全球不育患病率一直在上升，这主要是由于育龄晚期。有关女性正在寻找具有抗氧化特性的膳食补充剂，这被宣传为增加生育力的自然方法。姜黄素（CUR）是具有抗氧化剂和抗炎特性的多酚。如一些肿瘤细胞中所证明的，CUR引起凋亡细胞死亡。在这项工作中，研究了CUR对颗粒细胞（GC）的影响。GC围绕卵母细胞，提供其发育必需的养分交换和激素产生。使用来自接受辅助生殖技术（ART）的患者的COV434细胞系和原代人颗粒细胞（hGC）培养物。在不同时间（24-72小时）用CUR（0.001-50μM）处理GC。低浓度的CUR显示细胞活力增加。同样 它导致应激诱导后ROS / RNS的形成减少，表明具有保护作用。没有观察到激素水平的变化。相反，高浓度的CUR引发细胞活力降低和程序性的细胞死亡机制。与上述结果有关，高剂量的CUR影响了GC的激素功能。我们的工作在GC的主要功能上并因此在生殖成功上加强了膳食补充剂（即CUR）的益处。