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Cardiac function and blood flow hemodynamics assessment of zebrafish (Danio rerio) using high-speed video microscopy.
Micron ( IF 2.4 ) Pub Date : 2020-05-19 , DOI: 10.1016/j.micron.2020.102876
Fatiha M Benslimane 1 , Zain Z Zakaria 2 , Samar Shurbaji 1 , Mahmoud Khatib Ali Abdelrasool 1 , Mashael Ali H I Al-Badr 1 , Enas Said Khalil Al Absi 1 , Huseyin C Yalcin 1
Affiliation  

Background

In the last few decades, zebrafish (Danio rerio) were introduced as a model organism to investigate human diseases including cardiovascular and neuronal disorders. In most zebrafish investigations, cardiac function and blood flow hemodynamics need to be assessed to study the effects of the interference on the cardiovascular system. For heart function assessment, most important parameters include heart rate, cardiac output, ejection fraction, fractional area change, and fractional shortening.

Methods

A 10 s high-speed video of beating heart and flowing blood within major vessels of zebrafish that are less than 5 days post fertilization (dpf) were recorded via a stereo microscope equipped with a high speed camera. The videos were analyzed using MicroZebraLab and image J software for the assessment of cardiac function.

Results

Using the technique described here, we were able to simply yet effectively assess cardiac function and blood flow dynamics of normal zebrafish embryos. We believe that the practical method presented here will help cardiac researchers using the zebrafish as a model to examine cardiac function by using tools that could be available in their laboratory.



中文翻译:

使用高速视频显微镜评估斑马鱼 (Danio rerio) 的心脏功能和血流血流动力学。

背景

在过去的几十年里,斑马鱼 ( Danio rerio ) 被引入作为一种模式生物来研究人类疾病,包括心血管和神经元疾病。在大多数斑马鱼调查中,需要评估心脏功能和血流血流动力学,以研究干扰对心血管系统的影响。对于心脏功能评估,最重要的参数包括心率、心输出量、射血分数、面积变化分数和缩短分数。

方法

通过配备高速摄像机的立体显微镜记录了受精后不到 5 天的斑马鱼主要血管内跳动的心脏和血液流动的 10 秒高速视频。使用 MicroZebraLab 和 image J 软件对视频进行分析,以评估心脏功能。

结果

使用此处描述的技术,我们能够简单而有效地评估正常斑马鱼胚胎的心脏功能和血流动力学。我们相信,这里介绍的实用方法将帮助心脏研究人员使用斑马鱼作为模型,通过使用他们实验室中可用的工具来检查心脏功能。

更新日期:2020-05-19
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