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Microbes of XVI century Arrases of Krakow Royal Castle.
Microbiological Research ( IF 6.7 ) Pub Date : 2020-05-19 , DOI: 10.1016/j.micres.2020.126485
K Turnau 1 , R J Jędrzejczyk 2 , R Ważny 2 , D Chlebda 3 , M Janicka 1 , D Pawcenis 3 , T Łojewski 4
Affiliation  

Sixteenth-century Arrases from the Krakow Royal Castle constitute a Polish national cultural heritage; hence, they should be under special protection. The storage of historic objects at strict temperature and humidity conditions is fundamental for their protection against microorganisms. However, sometimes active preservation must be applied to disinfect bio-contaminated objects. In this study, 39 strains of microorganisms, both fungi and bacteria, isolated from Arrases deposited in the Wawel warehouse were isolated and then identified using molecular tools. Fungal ability to colonize and degrade silk and wool laboratory samples was studied. Selected microbial strains were compared concerning their ability to affect silk fibroin using size-exclusion chromatography (SEC). The effectiveness of low-temperature helium-generated plasma in disinfecting the model wool and silk samples inoculated with selected strains was tested. The results showed that plasma, despite its high biocidal activity in the case of paper, was not effective on porous textile materials. The alternative protection mechanism, involving active packaging in the form of textiles impregnated with silver-exchanged zeolites, could be used to separate layers of rolled tapestries in the warehouse. Nevertheless, optimal temperature and humidity conditions should be strictly maintained and controlled.



中文翻译:

十六世纪的微生物克拉科夫皇家城堡。

克拉科夫皇家城堡的16世纪Arrases构成了波兰的民族文化遗产。因此,它们应该受到特别保护。在严格的温度和湿度条件下存储历史遗物是其免受微生物侵害的基础。但是,有时必须进行主动保存以对生物污染的物体进行消毒。在这项研究中,从沉积在Wawel仓库中的Arrass中分离出39种微生物,包括真菌和细菌,然后使用分子工具对其进行鉴定。研究了真菌定殖和降解丝绸和羊毛实验室样品的能力。使用尺寸排阻色谱法(SEC)比较了选定的微生物菌株对丝素蛋白的影响能力。测试了低温氦气产生的等离子体对用选定菌株接种的模型羊毛和丝绸样品进行消毒的有效性。结果表明,尽管对纸具有很高的杀生物活性,但等离子体对多孔纺织材料无效。替代性保护机制包括以浸有银交换沸石的纺织品形式的主动包装,可用于分离仓库中卷起的挂毯层。但是,应严格保持和控制最佳温度和湿度条件。涉及以包装有浸银交换沸石的纺织品形式的主动包装,可用于分离仓库中卷起的挂毯层。但是,应严格保持和控制最佳温度和湿度条件。涉及以包装有浸银交换沸石的纺织品形式的主动包装,可用于分离仓库中卷起的挂毯层。但是,应严格保持和控制最佳温度和湿度条件。

更新日期:2020-05-19
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