BACKGROUND During an acute stroke, reactive oxygen species are overproduced and the endogenous antioxidative defense systems are disrupted. Therefore, antioxidative therapy can be a promising scheme to reduce the severity of stroke. Neumentix is a novel antioxidative supplement produced from a patented mint line and contains a high content of rosmarinic acid (RA). Although Neumentix has proven diverse efficacy and safety in clinical trials, its effect on strokes is unclear. METHODS Mice that were treated with Neumentix or vehicle for 14 days underwent transient middle cerebral artery occlusion (tMCAO) for 60 min. Mice were sacrificed 5 days after tMCAO. RESULTS Neumentix preserved body weight after tMCAO, showed a high antioxidative effect in serum, and reduced infarction volume compared to the vehicle. The expression of 4-hydroxy-2-nonenal, Nε-(carboxymethyl) lysine, and 8-hydroxy-2'-deoxyguanosine was reduced in Neumentix-treated mice. CONCLUSION The antioxidative effect of Neumentix was confirmed. This is the first report to demonstrate the antioxidative effect of Neumentix on strokes.

中文翻译：

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新型膳食补充剂Neumentix在小鼠中风模型中的抗氧化作用。

背景技术在急性中风期间，过量产生活性氧并且破坏了内源性抗氧化防御系统。因此，抗氧化疗法可能是减少中风严重程度的一种有前途的方案。Neumentix是由获得专利的薄荷系列生产的新型抗氧化补品，并且含有高含量的迷迭香酸（RA）。尽管Neumentix在临床试验中已证明了多种功效和安全性，但其对中风的影响尚不清楚。方法用Neumentix或媒介物治疗14天的小鼠经历短暂的大脑中动脉闭塞（tMCAO）60分钟。在tMCAO后5天处死小鼠。结果Neumentix保留了tMCAO后的体重，与载体相比在血清中显示出很高的抗氧化作用，并减少了梗塞体积。4-羟基-2-壬烯醛的表达 Neu-（羧甲基）赖氨酸和8-羟基-2'-脱氧鸟苷在Neumentix处理的小鼠中减少。结论证实了Neumentix的抗氧化作用。这是第一个证明Neumentix对中风具有抗氧化作用的报告。