Streptococcus equi ssp. zooepidemicus (SEZ) is an important swine pathogen and responsible for a wide variety of infections in many animal species. FabF was a novel protein identified in the previous study. However, its protective efficacy remained to be evaluated. In this study, recombinant fabF of SEZ was expressed and showed a strong immunoreactivity with mini-pig convalescent sera. Study in mice revealed that the recombinant protein induced a marked antibody response and protected 80% of mice against SEZ infection. The hyperimmune sera against fabF could efficiently kill the bacteria in the phagocytosis test. In addition, it was also found that anti- fabF antibodies can significantly inhibit the formation of SEZ biofilm. These study suggest that fabF may represent immunogens of interest for vaccine development against SEZ infection.

中文翻译：

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鉴定一种新型保护性抗原，马链球菌的3-氧代酰基-[酰基载体蛋白]合酶II。具有保护作用的动物流行病。

马链球菌 兽疫病（SEZ）是重要的猪病原体，对许多动物物种造成多种感染。FabF是先前研究中鉴定的一种新型蛋白质。但是，其保护功效尚待评估。在这项研究中，表达了SEZ的重组fabF，并显示出与小型猪恢复期血清的强免疫反应性。在小鼠中的研究表明，重组蛋白诱导了显着的抗体反应，并保护了80％的小鼠免于SEZ感染。针对fabF的超免疫血清可以在吞噬作用测试中有效杀死细菌。此外，还发现抗fabF抗体可以显着抑制SEZ生物膜的形成。这些研究表明，fabF可能代表针对SEZ感染的疫苗开发所关注的免疫原。